姜黄素对脂多糖诱导的尾核神经元损害的保护作用

Cyclooxgense-2 mediated antagonised effect of Curcumin on LPS induced impairment of neurons in caudate nucleus

目的探讨姜黄素(Curcumin,Cur)与环氧合酶-2(cyclooxgense-2,COX-2)对脂多糖(lipopolysaccride,LPS)诱导的尾核神经元损害的保护作用。方法以LPS损伤原代培养的尾核神经元为损伤的模型,在原代培养的尾核神经元给予LPS、姜黄素或COX-2的选择性抑制剂NS398联合应用LPS后,采用免疫印迹检测COX-2和NF-κB蛋白的表达及Hochest染色观察姜黄素及COX-2对LPS诱导的尾核神经元细胞凋亡的效应。结果①Hochest染色检测细胞核形态,LPS诱导尾核神经元表现出典型的凋亡特征,姜黄素或COX-2的选择性抑制剂NS398均可拮抗LPS诱导的尾核神经元损害的效应。②LPS可显著增强尾核神经元COX-2的表达水平,姜黄素或NS398均可抑制LPS诱导的尾核神经元COX-2的增强效应。③LPS可显著增强尾核神经元磷酸化的NF-κB蛋白的表达水平,该增强效应均可为姜黄素或NS398所拮抗。结论姜黄素对LPS诱导的尾核神经元损害的拮抗作用可能是通过COX-2介导的。

Objective The role of Curcumin and cyclooxygenase （COX -2） on lipopolysaccharide （LPS） induced impairment of neurons in caudate nucleus is evaluated. Methods Using the primary cultured neurons in caudate nucleus model with damage induced by LPS to observe the effect of Curcumin on LPS induced impairment of neurons in LPS - , Curcumin - or NS398 （ a selec- tive inhibitor of COX - 2 ） - treated cultured neurons in caudate nucleus was detected by Hochest staining and expressions of COX - 2 and phospho - NF - KB in LPS or Cureumin - treated cultured neurons were measured by immunoblotting technique. Results （~）Cur or NS398 inhibited the cellular apoptosis induced by LPS using Hochest stain. （~）Elevated COX -2 expression in LPS - treated primary cultured neuron in caudate nucleus was significantly suppressed by Curcumin. （~）Elevated expression of phospho - NF - KB in LPS - treated neurons in caudate nucleus was robustly inhibited by Curcumin or NS398. Conclusion Antagonised effect of Curcumin on LPS induced impairment of neurons in caudate nucleus might be mediated via COX - 2 signalling pathway.