玉米须水煎液对1型、2型糖尿病小鼠降血糖活性的研究

Hypoglycemic effect of corn silk decoction in alloxan- induced type 1 and type 2 diabetic mice

目的研究玉米须水煎液对四氧嘧啶所诱导的1型和2型糖尿病模型小鼠体重和血清中血糖(GLU)、总胆固醇(TC)、甘油三酯(TG)、超氧化物歧化酶(SOD)、丙二醛(MDA)、游离脂肪酸(NEFA)的影响。方法采用两次注射四氧嘧啶(150 mg·kg-1)和高脂饲料(10%蛋黄,10%猪油,1%胆固醇,0.2%胆盐,78.8%基料)结合低剂量单次注射四氧嘧啶(90 mg·kg-1)两种方法造模,建立1型、2型糖尿病小鼠模型。玉米须水提取物18 g·kg-1(相当于生药材重量)的剂量灌胃给药,4周后,测定小鼠的空腹血糖值、糖耐量及血液各项生化指标。结果玉米须水煎液降低了1型、2型糖尿病小鼠GLU、TC、TG、NEFA和MDA水平,升高了1型和2型糖尿病小鼠SOD水平。结论玉米须水煎液能缓解1型糖尿病小鼠的高血糖症状,降低2型糖尿病小鼠的血糖值,并可调节其血脂异常。

Objective To investigate the effect of corn silk decoction on the levels of serum GLU, TC,TG, SOD, MDA and NEFA in alloxan - induced diabetic mice. Methods Type I diabetic mice were induced by a twice high - dose （ 150 mg/kg） fresh allox- an solution and were subject to intraperitoneal injection at an interval of two days. Type II diabetic mice were induced by a high - fat diet （ 10% yolk, 10% lard oil, 1% cholesterol,0.2% bile salt and 78.8 % base stock） for one month followed by intraperito- neal injection of alloxan at 90 mg ~ kg-1. Then they were treated by intragastric administration of corn silk decoction at the dosage of 18 g/kg for 4 weeks. The anti - diabetic effects were observed by determining the levels of fasting blood glucose,glucose tolerance and other blood biochemical indices. Results Corn silk decoction could reduce the levels of GLU, TC, TG and MDA. Also, the activity of SOD was increased in alloxan - induced typl and type 2 diabetic mice after treated with corn silk decoction for 4 weeks. Conclusion 4 weeks＇treatments of corn silk decoction could alleviated the blood glucose level and other symptoms of diabetes in type I diabetic mice. Moreover,corn silk decoction could significantly reduce blood glucose level and improve lipid metabolism in type lI diabetic mice.