山蜡梅颗粒剂中4种有效成分含量的反相高效液相色谱法同时测定

Simultaneous determination of the content of four active compounds in Shanlamei Granules by RP- HPLC

目的建立反法同时测定山蜡梅颗粒剂中4种有效成分的含量。方法采用Elitehypersil ODS2（4．6mm×250mm，5μm）色谱柱，流动相为乙腈一水，梯度洗脱，流速1．0ml／min，检测波长344nm，柱温为40℃。结果东莨菪内酯在5．665～56．65μg／ml浓度范围内线性关系良好，r=0．9999，平均回收率为94．77％，RSD为1．27％（n=6）；异嗪皮啶在2．4312—24．312μg／ml范围线性关系良好，r=0．9999，平均回收率为102．86％，RSD为0．93％（n=6）；滨蒿内酯在2．444～24．44μg/ml范围线性良好，r=0．9999，平均回收率为102．18％，RSD为1．81％（n=6），芦丁在9．012～90．12μg/ml范围线性良好，r=0．9992，平均回收率为104．44％，RSD为4．2％（n=6）。结论该方法操作简便，结果准确，重复性好，可用来控制山蜡梅颗粒剂的质量。

Objective To set up the method for simultaneous determination the content of four active compounds in Shanlamei Granules by HPLC. Methods The chromatography separation was performed on the Elite hypersil ODS2 （4.6 mm × 250 mm,5 μm） with gradient elution. The mixture of acetonitrile and water as the mobile phases at a flow rate of 1.0 ml·min^-1 was used, the detective wavelength was 344 nm and the column temperature was 40 ℃. Results The calibration curve was linear in the range of 5. 665 - 56.65 μg/ml for seopoletin （ r = 0. 9999 ） ,the average recovery was 94.77% and RSD was 1.27%. The calibration curve was linear in the range of 2. 4312 - 24. 312 μg/ml for isofraxidin （r = 0.999 9 ） ,the average recovery was 102.86% and RSD% was 0.93 %. The standard curve presented a linear range from 2. 444 - 24.44 μg/ml for scoparone （ r = 0. 9999） , the average recovery was 102.18% and RSD was 1.81%. The standard curve presented a linear range from 9. 012 - 90.12μg/ml for rutin （ r = 0. 9992 ）, the average recovery was 104.44% and RSD was 4.2%. Conclusion The method was available with a good reproducibility, sensitive and simple and could be used to control the quality of Shanlamei Granules.