错配修复基因MSH5多态性与非小细胞肺癌的易感性研究

Association of MSH5 gene C85T polymorphism with susceptibility of non-small-cell lung carcinoma

目的探讨错配修复基因MSH5C85T多态性与非小细胞肺癌（non-small—eelllungcarcinoma，NSCLC）易感性的相关性。方法采用PCR一限制片段长度多态性分析技术（PCR—RHP），对中国人群107例NSCLC患者和120名健康体检者进行候选基因MSH5C85T多态性分析，后期随机抽取50％标本进行测序验证。结果NSCLC组MSH5基因C85T多态性CC、CT、1vr基因型频率分别为59．8％、35．5％、4．7％，对照组分别为78．3％、20．0％、1．7％，NSCLC组CT、TT基因型频率高于对照组（x2=9．170，P=0．002；OR=1．855，95％CI=1．229～2．798）；NSCLC组C／T等位基因频率分别为77．6％、22．4％，对照组分别为88．3％、11．7％，NSCLC组T等位基因频率高于对照组（x2=9．405，P=0．002；OR=1．923，95％C1=1．253～2．950）；后期随机抽取的50％标本测序结果与RFLP完全相符。结论在中国人群中，MsH5C85T多态性与NSCLC易感性呈显著相关。

Objective To explore the association of MSH5 gene C85T polymorphism with the susceptibility of non-small-cell lung carcinoma （NSCLC）. Methods The genotyping was performed by using the PCR-RFLP method in 107 N~：I2LC cases and 120 controls. Then, 50% samples were selected randomly to be sequenced. Results The MSH5 gene C85T polymorphism, the frequencies of CC, CT and TI＂ were 59.8 %, 35.5 %, 4.7 % in the NSCLC group, and 78.3 %, 20.0 %, 1.7 % in the normal control group, respectively. The frequency of the CT ＋ TT genotype in NSCLC was significantly higher than that in the controls （？（2 = 9. 170, P = 0. 002, OR = 1. 855,95 % CI = 1. 229--2. 798）. The frequencies of the two alleles C and T were 77.6% and 22.4% in the NSCLC group, and were 88.3% and 11.7% in the normal control group, respectively. The T allele frequency was significantly higher in the NSCLC group than in the controls （~.2 = 9. 405, P = 0. 002; OR = 1. 923,95% CI = 1. 253 --2. 950）. The results of sequencing were completely consistent with those obtained by the PCR-RFLP method. Conclusions MSH5 C85T polymorphism is associated with the susceptibility of NSCLC significantly in a Chinese population.