布拉氏酵母菌对肝硬化模型大鼠的影响

The influence of Saccharomyces cerevisiae boulardii on rats model of cirrhosis

目的研究布拉氏酵母菌能否通过改善肠源性内毒素血症、肠道环境改善四氯化碳诱导的肝硬化模型大鼠肝纤维化的程度。方法 50只雄性Wistar大鼠随机分为正常组(8只)、模型组(20只)、预防组(14只)和治疗组(8只)。预防组在制模同时给予喂服布拉氏酵母菌制剂,治疗组在制模成功后开始给予喂服布拉氏酵母菌制剂,正常组和模型组给予同等生理盐水喂服。实验过程中每周称量所有大鼠体重,观察其日常生活习性,实验在18周末处死所有大鼠,HE染色观察肝脏病理改变,测定血清中天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)、丙二醛(MDA)和白蛋白(ALB)水平及血浆内毒素含量,用变性梯度凝胶电泳法检测大鼠肠道菌群情况。结果正常组[(0.052±0.005)EU/mL]、预防组[(0.058±0.028)EU/mL]和治疗组[(0.230±0.027)EU/mL]大鼠血浆中的内毒素明显低于模型组[(0.310±0.039)EU/mL](P<0.05)。预防组和正常组大鼠血浆内毒素含量有区别,但差异无统计学意义。通过变性梯度凝胶电泳发现正常组大鼠肠道菌群数量明显好于模型组,肝硬化大鼠肠道菌群失衡。而治疗组介于预防组和模型组之间。结论布拉氏酵母菌对于改善肝硬化模型大鼠肠道菌群情况,减少肠源性内毒素血症有重要意义。

Objective Saccharomyces boulardii could be passed to improve the long-borne endotoxemia, intestinal environment to improve the degree of cirrhosis rat model of liver fibrosis induced by carbon tetrachloride. Methods 50 male Wistar rats were randomly divided into four groups：normal group, model group, prevention group and treatment group. Prevention and treatment groups, respectively, starting from different points in time given to feed- ing Saccharomyces boulardii preparations. The weekly weighing all the body weight of rats during the experiment, observe their daily living habits, experiments all rats were killed on the 18th weekend, HE staining of liver patho- logical changes. Determination of serum aspartate aminotransferase （AST）, alanine aminotransferase （ALT）, ma- londialdehyde （MDA） and albumin （ALB） and the level of plasma endotoxin content were detected using denatu- ring gradient gel electrophoresis. Results Normal group （0.052 ± 0. 005） EU/mL, prevention group （0. 058 ± 0. 028） EU/mL and treatment group （0. 230 ± 0. 027 ） EU/mL in rat plasma endotoxin were significantly lower than the model group （0.310 ＋ 0. 039 ）EU/mL （P 〈 0.05 ）. Prevention group and the normal group rat plasma endotox- in levels were different, but had not statistically significant. Found that the number of normal rats intestinal flora was significantly better than the model group, cirrhosis of the liver in rat intestinal flora imbalance by denaturing gradient gel electr@oresis. Treatment group between the prevention group and model group. Conclusion Saccharomyces cerevisiae have important significance for improve intestinal flora on rats model of cirrhosis and reduce intestinal source endotoxemia.