替吉奥对BALB/c小鼠肠道菌群的影响

The effects of S-1 Capsules on the intestinal microbiota of BALB /c mice

目的应用PCR-DGGE方法研究抗癌剂替吉奥对肠道菌群的影响。方法取BALB/c小鼠10只,灌服替吉奥(441 mg/kg)7 d。应用PCR-DGGE方法获得肠道菌群分子指纹图谱,进行相似性、多样性分析及优势条带的序列分析。结果实验0 d与实验7 d的小鼠肠道菌群结构差异存在统计学意义(P<0.01)。结论替吉奥能够杀灭肠道中的有益菌,促使致病菌过度生长,导致肠道菌群严重失调。

Objective To study the effects of S-1 on the intestinal microbiota of BALB/c mice by PCR-DGGE technique. Methods Ten BALB/c were chosen, water-soluble S-1 was given to mice at 441 mg/kg-1 of body weight seven days. Then the feces were collected at 0 d, 7 d. After extraction of bacterial genomic DNA from feces, the diversity, similarity analyses and the sequence analysis of the different bands were carried out bv DGGE profilings with PCR-DGGE （ p@merase chain reaction-denaturing gradient gel electrophoresis） technique. Results The in- testinal microbiota composition and structure have very obvious difference between these feces （ P 〈 0.01 ）. Conclusion S-1 can destroy intestinal microeeology, killing probiotics and creating a breeding ground for patho- genic bacterium.