溶剂置换结晶法制备甘露糖的研究

On Preparation of Mannose with Solvent Replacement Crystallization Method

[目的]介绍一种制备高纯度D-甘露糖晶体的方法.[方法]以甘露糖、葡萄糖混合溶液为原料,有机溶剂置换结晶法得到粗晶产品,乙醇浸泡洗涤晶体得到纯度在99％以上的甘露糖晶体.通过比较筛选适合的置换有机溶剂,在此基础上,考察了乙醇的用量、乙醇浸泡洗涤的时间对高纯度D-甘露糖晶体得率的影响.[结果]试验确定了置换溶剂乙醇的用量以及乙醇浸泡洗涤的时间：以乙醇为结晶置换溶剂,最佳用量为60％,以乙醇为洗涤溶剂,最佳浸泡洗涤时间为20～30 min,最终甘露糖纯度达到99％以上,收率60％以上.[结论]溶剂置换结晶法制备高纯度D-甘露糖晶体的结晶时间短、能耗低、操作简单.

[ Objective ] To introduce a method for preparation of high purity D-mannose crystal. [ Method ] With mixed solution of mannose and glucose as raw material, rough crystals were obtained by using organic solvent replacement. After washing by alcohol, mannose crystal a- bove 99% purity was obtained. The optimal organic solvent was selected, on the basis of this, effects of optimal dosage and soaking time of al- cohol on yield of high purity D-mannose crystals were investigated. [ Result ] With alcohol as replacement solvent, the optimal dosage is 60% ; with alcohol as washing solvent, the optimal soaking time is 20 -30 rain, the purity and yield of mannose could reach above 99% and 60% , respectively. [ Conclusion] Preparation of D-mannose crystal with solvent replacement crystallization method has advantages of simple, short time and low consumption.