ApoA5基因单核苷酸多态性与代谢综合征的相关性

Relationship between ApoA5 gene polymorphisms and metabolic syndrome

目的探讨ApoA5基因单核苷酸多态性与代谢综合征的关系。方法随机选取2012年1月至2013年1月在我校附属医院接受治疗的100例代谢综合征(MS)患者,并采用1:1匹配设计选取100例正常人为研究对象。采用PCR-RFLP分析ApoA5基因-1131T>C和56C>G两个SNP位点单核苷酸多态性与代谢综合征的关系。结果 ApoA5-1131T>C CC基因型携带者的TG、HDL-C、ApoA1的水平明显高于TC、TT基因型携带者,差异有统计学意义(P<0.05)。而ApoA5 56C>G各基因型之间MS患者的临床及生化指标差异均无统计学意义(P>0.05)。ApoA5-1131T>C TC基因型携带者比CC基因型携带者更容易发生MS,风险分别上升1.415倍,而TT型基因携带者发生MS的风险为CC型的0.302倍(P<0.05)。结论 ApoA5-1131T>C的基因多态性与MS的发生密切相关,可增加MS发病率,而ApoA5 56C>G的基因多态性与MS的发生无显著相关。

Objective To explore the relationship between metabolic syndrome and ApoA5 gene polymor- phism. Methods One hundred patients with metabolic syndrome （MS） matched with 100 healthy subjects were se- lected randomly from January 2012 to January 2013 in the Affiliated Hospital of Huaihua Medical College. PCR-RFLP was used to analyze if there was a correlation between the single nucleotide polymorphisms of ApoA5 gene-ll31T〉C and 56C〉G SNP and metabolic syndrome. Results The levels ofTG, HDL-C, ApoA1 of subjects with ApoA5-1131T〉C CC genotype were significantly higher than those of subjects with TC and TT genotype （P〈 0.05）. The clinical and biochemical parameters showed no significant difference between MS patients with ApoA5 56C〉G genotypes （P〉0.05）. ApoA5-1131T〉C TC genotype increased the risk of developing MS by 1.415, but TT gcno- type decreased the risk of developing MS comparing to CC genotype by 0.302 （P〈0.05）. Conclusion The SNP of ApoA5-1131T〉C is closely related to the occurrence of MS and can increase the risk of MS, while SNP of ApoA5 56C〉G has no relationship with the occurrence of MS.