应用Mab-basedAC-ELISA法对鸡IBDV分离株的抗原特性分析

Antigenic characteristic analysis of chicken IBDV isolates with Mab-based AC ELISA

为了解传染性法氏囊病病毒(IBDV)的抗原变异分子基础,采用Mab-based AC-ELISA方法,利用11株单抗对2株河北IBDV分离株进行了抗原特性分析,并比较了超强毒株和弱毒株的VP2高变区序列的分子特征。结果显示:强弱毒株的抗原位点结合单抗的能力明显不同,除了Mab3、Mab9外,其余9个单抗的结合能力,超强毒株要高于弱毒株。超强毒株HeB-lf缺少了Mab3结合位点,弱毒株HeB-bdjz缺少了Mab3、Mab6结合位点,这说明强弱毒株不仅在序列上有差异,而且在抗原位点的缺失上也存在差异。本试验为控制IBD和疫苗改进具有重要意义。

In order to understand molecular basis of antigenic variation on infectious bursal disease virus,antigenic characterstics of 2 IBDV isolates from Hebei province were analyzed to bind 11 Mabs with monoclonal antibody-base antigen capture ELISA（Mab-based AC-ELISA） in the test. And molecular characteristics of VP2 hypervariable regions were compared between very virulent isolates and attenuated isolates. The results showed that it is obviously distinct on ability of anti- gen site binding Mab. Apart from Mab3 and Mab9,in antigen capture ability of other 9 Mabs very virulent IBDV（vvIBDV）isolates are higher than that of attenuated isolates. Mab3 antigen-bind site was not detected in Hebei-lf isolates,while Mab3 and Mab6 site were both detected in HeB-bdjz i- solates. This suggested that it is different in the sequence and antigen site missing between very virulent IBDV and attenuated isolates.