沙冬青总生物碱体外抗牛病毒性腹泻病毒

Effect of total alkaloids in ammopiptanthus mongolicus on BVDV in vitro

为了探讨沙冬青总生物碱抗牛病毒性腹泻病毒的作用,采用药毒同加、先药后毒、先毒后药3种不同途径进行体外抗病毒试验,观察病毒致牛肾原代细胞病变,并采用MTT比色法测定D492值,计算病毒抑制率。结果表明,病毒的TCID50为10-5.29/0.1mL;沙冬青种子总生物碱的药物安全质量浓度为0.39g/L;与病毒对照组相比,当质量浓度为0.16g/L和0.32g/L时,沙冬青种子总生物碱对牛病毒性腹泻病毒有较强的直接杀伤作用,可抑制病毒的复制,能有效保护经病毒感染的牛肾原代细胞,使细胞活性增强,并减弱病毒导致的细胞病变效应,但对病毒没有明显的阻断作用。综上所述,沙冬青种子总生物碱具有较强的抗牛病毒性腹泻病毒的作用。

In order to investigated anti-virus mechanism of the total alkaloids from seeds of am- mopiptanthus mongolicus against bovine viral diarrhea virus （BVDV）. Cytopathic effect（CPE） of Madin-Darby bovine kidney（MDBK） induced by BVDV was observed and D49z value was assayed by three administration routes of virus and drug meanwhile, virus after drug, virus before drug. The results showed that BVDV TCID50 is 10^-5.29/0. lmL. The drug safety concentration is 0.39 g/L;compared with the virus control group,the total alkaloids of seeds from ammopiptan- thus mongolicus （0.16 g/L, 0. 32 g/L）could directly kill BVDV, inhibit the reproduction of BVDV, protect MDBK infected by BVDV,increase the cells proliferated activity,and inhibit the cy- topathic effect. But the blocking effect of the total alkaloid on BVDV was not observed. In conclu- sion, the total alkaloids from seeds of ammopiptanthus mongolicus has remarkable antiviral effect.