芪菊袋泡茶抗菌及抗病毒的药效学研究

Pharmacodynamics Study of Antibacterial and Antiviral Effects of Qiju Tea Bags

目的探讨芪菊袋泡茶的抗炎、抑菌及抗病毒的作用。方法以小鼠二甲苯所致耳廓肿胀及腹腔毛细血管通透性实验,观察芪菊袋泡茶的抗炎作用,以定量测定稀释法观察芪菊袋泡茶对金黄色葡萄球菌、乙型溶血性链球菌、肺炎链球菌的抑制作用;以鸡胚法探讨芪菊袋泡茶对H1N1流感病毒的抑制作用。结果芪菊袋泡茶低、中剂量组及鼻咽清毒颗粒组,对小鼠耳廓肿胀度的抑制作用与空白对照组比较,差异均有统计学意义(P<0.01);及对小鼠腹腔毛细血管通透性的抑制作用与模型组比较,差异有统计学意义(P<0.05,P<0.01);芪菊袋泡茶对金黄色葡萄球菌最小抑菌浓度为0.4 g·mL-1,对乙型溶血性链球菌最小抑菌浓度为0.2 g·mL-1,对肺炎链球菌最小抑菌浓度为0.4 g·mL-1,其体外抑菌效果优于对照药鼻咽清毒颗粒;与模型组比较,100mg·mL-1浓度芪菊泡袋茶组鸡胚尿囊液血凝价显著降低,差异有统计学意义(P<0.05),100 mg·mL-1以下浓度对H1N1流感病毒在鸡胚体内的复制无显著影响,差异无统计学意义(P>0.05)。结论芪菊袋泡茶有体内抗炎以及体外抑菌、抗病毒作用,该结果可为芪菊袋泡茶工艺生产提供药理学依据。

Objective To explore the anti-inflammatory and anti- bacteriostasis effects of Qiju tea hags. Methods By observing the auricle swelling caused hy dimethylbenzene in mice and the effect on blood capillary permeability of mice abdominal cavity, the anti-inflammatory effect of Qiju tea bags was explored. Dilution method and quantitative determination was used to observe the bacteriostasis of Qiju tea bags on Staphylococcus aureus, hemolytic streptococcus beta and Streptococcus pneumoniae. By applying the chicken embryo method, the antivirus effect ~ff Qiju tea bags on H1N1 flu virus was examined. Results The low- and middle-dose Qiju tea bags and Biyan Qingdu particles had inhibitory effect on the auricle swelling in mice and the abdominal blood capillary permeability, the difference being significant when compared with the blank controlled group（P 〈 0.05）. The minimal inhibitory concentration of Qiju tea bags was 0.4 g ＂mL-t for Staphylococcus aureu, 0.2 g ＂mLI for beta hemolytic streptococcus and 0.4 g ＂mL for Streptococcus pneumoniae, the bacteriostasis of Qiju tea bags being better than that of the control medicine Biyan difference was shown in the reproduction of the H1N1 in the chick embryo when the concentration of Qiju tea bags was below 100 mg＇mL-t（P 〉 0.05）. Conclusion Qiju tea bags have anti-inflammatory in vivo and have bacteriostasis and antiviral effect in vitro, and the experimental results will supply pharmacological evidence for the technological production of Qiju tea bags.