几种物质对亚硝酸盐清除作用的比较

Comparisons of nitrite scavenging from several substances

采用对氨基苯磺酸-盐酸萘乙二胺分光光度法测定了抗坏血酸(V C)、儿茶酚、葡萄糖、牛血清白蛋白4种物质对亚硝酸盐的清除率。在单因素实验基础上,确定了上述几种化合物清除亚硝酸盐的影响因素(清除剂浓度,作用时间,反应温度及反应液pH)。然后采用均匀实验设计,考察在不同处理方式下它们对亚硝酸盐的清除率。结果表明,V C浓度0.7%、反应时间为15min、pH4、温度为4℃时,对亚硝酸盐清除效果最好;儿茶酚浓度为1%、反应时间为40min、pH2、反应温度为20℃时,其对亚硝酸盐清除效果最好;而葡萄糖、牛血清白蛋白对亚硝酸盐的清除率不到10%,没有显著的清除效果。

The scavenging capability of several substances such as ascorbic acid, catechol, grucose and Bovine serum arbumin （BSA）on nitrite was studied by aniline-suffonic and hydrochloride acid- napHthylamine ethylenediamine measuring through spectropHotometry.Based on the single factor experiment that explored the common effect on the scanvenging rate of nitrite, a uniform design test were designed to identify the best conditions for these substances to nitrite scanvenging. The results showed that the nitrite scanvenging effect reached upmost while ascorbic concentration 0.7%, reaction time 15min, pHA and temperature 4℃, respectively; Meantime,for catechol, its best scanvenging rate occured while same circumstance was 1%, 40rain, pH2,20℃ respectively. Besides, our experiment data testified that glucose and BSA perfomed less than 10% nitrite scanvenging.They had not a significant scanvenging effect on nitrition.