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启动子P_(43)表达关键酶基因对subtilosin A合成的调控作用 被引量：1

Promoter P_(43) regulatory effect for subtilosin A synthesis by expressing key enzyme gene
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摘要 为提高枯草芽孢杆菌168(Bacillus subtilis 168)细菌素subtilosin A的产量使其能够应用到食品防腐、医药卫生等行业。采用重叠延伸PCR将强启动子P43分别和subtilosin A合成酶基因簇中关键酶基因sbo-albA、albB-albC连接到一起,构建了高表达载体。采用改进的电击法将重组载体转化到Bacillus subtilis 168中,通过高效液相色谱(HPLC)检测,确定subtilosin A产量得到了提高,进一步通过抑菌实验证明其抑菌活性也获得了提高。该方法为提高subtilosin A产量,进而应用到生产实践中提供了理论参考。 For improving Bacillus subtilis 168 bacteriocin subtilosin A production and applying to food preservation, medical science and so on,P43was linked to key enzyme gene sbo-albA,albB-albC of subtilosin A operon using SOE-PCR：High expressing vector was constructed.This recombinant vector was transformed into Bacillus subtilis 168 by high osmolarity electroporation method.HPLC assay showed subtilosin A production was improved.Inhibited method also showed the improving.This method will provide theory reference of improving subtilosin A production and applying in productive practice.
作者 孙会刚 张充 吕凤霞 别小妹 陆兆新
机构地区 南京农业大学食品科技学院 徐州工程学院食品(生物)工程学院
出处 《食品工业科技》 CAS CSCD 北大核心 2013年第23期164-167,171,共5页 Science and Technology of Food Industry
基金 国家自然科学基金(31271936) 河北省自然科学基金资助项目(C2007000191) 徐州市科技计划项目(XF11C056) 徐州工程学院科研项目(SPY2010209)
关键词 启动子 subtilosin A 关键酶 调控 promoter subtilosin A key enzyme regulation
分类号 TS202.3 [轻工技术与工程—食品科学]
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参考文献14

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同被引文献11

  • 1胡世界,罗素兰,张吉贞,长孙东亭.巴斯德毕赤酵母表达系统及其高水平表达策略[J].生物技术,2007,17(6):78-83. 被引量：17
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  • 9Liu quanli,Gao guang,Xu haijin,et al. Identification of tile bacteriocin subtilosin A and loss of purt results in its high-level production in Bacillus amyloliquefaeiens[J]. Research in Microbiology, 2012, 163 ( 6 ) : 470-478.
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食品工业科技
2013年 第23期

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