大孔弱酸性离子交换树脂吸附分离庆大霉素的性能研究

Study on adsorption and separation of gentamicin by macroporous weakly acidic ion exchange resin

目的:探讨大孔弱酸性离子交换树脂Amberlite FPC3500吸附分离庆大霉素的性能。方法:在单因素实验基础上采用正交实验设计,研究FPC3500对庆大霉素粗品溶液的静态吸附、动态吸附及解吸的最佳条件,以由紫外分光光度值计算得到的吸附率、解吸率、回收率为考察指标。结果:静态吸附最佳时长为1h,pH7.0,室温,转速140r/min。在pH为7.6,样品浓度为5.64g/L,进样流速为0.6mL/min,氨水浓度为7%时庆大霉素的回收率可达94.80%。结论:大孔弱酸性树脂适用于庆大霉素的分离提纯,且吸附性能优于国内常用的凝胶型强酸性树脂。

Objective： In the paper, the process conditions of adsorption and separation of gentamicin by macroporous weakly acidic resin Amberlite FPC3500 were studied.Methods：Through static or dynamic adsorption and dynamic desorption experiments of resin, the preferable technical conditions of reclaiming gentamicin by FPC3500 resin were studied by using single factor experiment and orthogonal experiment,taking the adsorption rate,desorption rate and recovery ratio as the investigation index.Results ：the optimal condition of static adsorption was at pHT.0 with lh, room temperature, rotating rate of 140r/min.The recovery ratio was up to 94.8% at pH7.6, concentration of sampling 5.64g/L, flow rate of sampling 0.6mL/min, concentration of ammonia 7%.Conclusion： Macroporous weakly acidic resin was suitable for the separation and purification of gentamicin, adsorption capability was better than the commonly-used strong acid resin of gel type in our country.