1003株阴道分泌物真菌培养与结果分析

Fungal culture and result analysis of 1 003 vaginal discharge samples

目的:了解海宁市女性生殖道真菌感染率和选用有效抗真菌药物。方法:对海宁市人民医院妇科门诊就诊患者1 410例阴道分泌物标本用科玛嘉显色培养基进行培养,采用生物梅里ATB-FUNGUSTS试条及ID32C进行真菌鉴定和药敏试验。结果:1 410例分泌物标本中共培养出真菌1 003株,真菌阳性率达71.1%,以生育龄妇女为主,其中白色念珠菌感染率为92.3%。药敏试验所选5种抗真菌药物(5-氟胞嘧啶、两性霉素B、氟康唑、伊曲康唑、伏力康唑),其中两性霉素B和5-氟胞嘧啶具有较高的抗菌活性。结论:白色念珠菌仍是引起女性生殖道感染的主要真菌;真菌对大多数抗真菌药物具有不同程度的耐药性,所以微生物室应加强真菌耐药性监测,为临床提供用药依据,指导临床合理用药。

Objective： To understand the rate of fungal infection in vaginal discharge samples and how to choose effective antifun- gal drugs among women in Haining city. Methods： A total of 1 410 vaginal discharge samples from the female patients treated in department of gynecology in the hospital were cultured with CHROMagar culture medium, fungal identification and drug sensitive test were conducted with ATB -FUNGUSTS and ID32C. Results： Among 1 410 vaginal discharge samples; a total of 1 003 fungal strains were cultured, the positive rate of fungus was 71.1%, most of the women were at childbearing age, the infection rate of Candida albicans was 92. 3% ; five anti- fungal drugs were adopted in drug sensitive test （5 -Flurocytosine, Amphotericin, Fluconazole, Itraconazole, Voficonazole）, Amphotericin and 5 - Flurocytosine had relatively high antifungal activity. Conclusion： Candida albicans is still the major fungus inducing female genital tract infection; fungus has drug resistance to most antifungal drugs in varying degrees, so the drug resistance monitoring of fungus should be strengthened in clinical microbiology laboratory in order to provide reference for drug therapy and direct reasonable application of antifungal drugs.