期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

滋养细胞中STAT3活性与MMP-9表达水平的相关性 被引量：2

Correlation between STAT3 activity and MMP-9 expression level in trophoblast cells
原文传递
导出
摘要 目的:探讨滋养细胞中STAT3信号通路与侵袭性相关因子MMP-9的相关性。方法:应用免疫印迹实验、荧光定量PCR实验分析人绒毛膜滋养层细胞系JEG-3细胞中STAT3活性与MMP-9的表达水平。结果:SD-1008可特异性降低JEG-3中STAT3的磷酸化水平(0.35±0.14倍,Z=-2.201,P=0.028);与对照组相比,SD-1008处理组细胞中MMP-9mRNA(0.48±0.21倍,t=6.104,P=0.002)和蛋白(0.44±0.19倍,t=7.259,P=0.001)的表达水平均降低。结论:STAT3可能作为胞内信号通路的上游因子调节滋养细胞中MMP-9的表达。 Objective： To explore the correlation between STAT3 signal pathway and matrix metalloproteinase -9 （MMP -9） in trophoblast cells. Methods： Western blot and fluorescent quantitative polymerase chain reaction were used to analyze STAT3 activity and MMP-9 expression level in human chorionic JEG- 3 trophoblast cells. Results： SD -1008 reduced the phosphorylation level of STAT3 specifically by （0. 35 ±0. 14） fold （Z = -2. 201 , P =0. 028） ; compared with control group, the expression levels of MMP -9 mRNA and protein in SD- 1008 treatment group decreased by （0. 48 ±0. 21 ） fold and （0.44 ± 0. 19） fold, respectively （t = 6. 104, P = 0. 002; t = 7. 259, P = 0. 001 ） . Conclusion： STAT3 can regulate the expression of MMP - 9 in trophoblast cells as a upstream regulator of intracellu- lar signal pathway.
作者 张展 刘丽莎 高峻峻 张琳琳 贾莉婷 李莹 于海洋
机构地区 郑州大学第三附属医院检验科
出处 《中国妇幼保健》 CAS 北大核心 2013年第34期5709-5711,共3页 Maternal and Child Health Care of China
基金 河南省科技创新人才计划项目资助基金〔124200510005〕
关键词 滋养细胞 SD-1008 侵袭力 STAT3 金属基质蛋白酶-9 Trophoblast cell SD - 1008 Invasiveness STAT3 Matrix metalloproteinase - 9
分类号 R714 [医药卫生—妇产科学]
  • 相关文献

参考文献10

  • 1Hannon T, Innes B, Lash G, et al. Effects of local decidua on trophoblast invasion and spiral artery remodeling in focal placenta creta -an immunohistochemical study [ J . Placenta, 2012, 33 (12): 998 -1004.
  • 2Nissi R, Talvensaari -Mattila A, Kotila V, et al. Circulating matrix metalloproteinase MMP- 9 and MMP- 9/TIMP- 2 complex are associated with spontaneous early pregnancy failure [J] . Reprod Biol Endocrinol, 2013, 11 : 2.
  • 3Tian W, Li B, Zhang X, et al. Suppression of tumor invasion and migration in breast cancer cells following delivery of siRNA against Stat3 with the antimierobial peptide PR39 [J] . Oneol- ogy Reports, 2012, 28 (4): 1362-1368.
  • 4顾芸,李凤山,王小青,张翔,李青,武卫平.基质金属蛋白酶及其组织抑制物在胎盘、血浆中的表达及其意义[J].中国妇幼保健,2010,25(24):3475-3477. 被引量：5
  • 5Gremlich S, Nguyen D, Reymondin D, et al. Fetal MMP2/ MMP9 polymorphisms and intrauterine growth restriction risk [J] . J Reprod Immunol, 2007, 74 (1 -2): 143 -51.
  • 6Fitzgerald JS, Germeyer A, Huppertz B, et al. Governing the invasive trophoblast: current aspects on intra- and extracellu- lar regulation [J] Am J Reprod Immunol, 2010, 63 (6) : 492 - 505.
  • 7赵义清,章汉旺.STAT3在小鼠胚胎围着床期子宫内膜的表达与活化[J].中国妇幼保健,2007,22(13):1800-1802. 被引量：5
  • 8Duan Z, Bradner J, Grecnberg E, et al. 8 - benzyl - 4 - oxo - 8 - azabicyclo [ 3.2. 1 ] oct - 2 -ene - 6, 7 - dicarboxylic acid (SD - 1008), a novel janus kinase 2 inhibitor, increases chemotherapy sensitivity in human ovarian cancer cells [J] . Mol Pharmacol, 2007, 72 (5) : 1137 -45.
  • 9Wang XH, Liu BR, Qu B, et al. Silencing STAT3 may inhib- it cell growth through regulating signaling pathway, telomer- ase, cell cycle, apoptosis and angiogenesis in hepatocellular carcinoma: potential uses for gene therapy [ J . Neoplasma, 2011, 58 (2): 158-71.
  • 10Liu X, Gu W, Li X. HLA - G regulates the invasive proper- ties of JEG - 3 choriocarcinoma cells by controlling STAT3 ac- tivation [J] .Placenta, 2013, 34 (11): 1044-52.

二级参考文献15

  • 1李向红,马海燕,王晓燕.妊娠期基质金属蛋白酶的调控及其与胎盘发育的关系[J].中国医药生物技术,2007,2(4):310-313. 被引量：6
  • 2易旺军,王新.基质金属蛋白酶-2及其组织抑制剂-2在妊娠期高血压疾病患者胎盘中的表达[J].南华大学学报（医学版）,2007,35(1):34-37. 被引量：2
  • 3Bischof P, Meisser A, Campana A. Biochemistry and molecular biology of trophoblast invasion [J] . Ann N Y Acad Sci, 2001, 943:157.
  • 4Staun - Ram E, Goldman S, Gabarin D et al. Expression and importance of matrix tnetalloproteinase 2 and 9 ( MMP - 2 and - 9 ) in human trophoblast invasion [ J]. Reprod Biol Endocrinol, 2004, 2 : 59.
  • 5Goldman S, Shalev E. The role of the matrix metalloproteinases in human endometrial and ovarian cycles (J) . Eur J Obstet Gynecol Reprod Biol, 2003, 111 (2): 109.
  • 6Fata JE, Ho AT, Leco KJ et al. Cellular turnover and extracellular matrix remodeling in female reproductive tissues : functions of metalloprotei- nases and their inhibitors [J] . Cell Mol Life Sci, 2000, 57 ( 1 ) : 77.
  • 7Catalano RD,Johnson MH,Campbell EA et al.Inhibition of Stat3 activation in the endometrium prevents implantation:a nonsteroidal approach to contraception.Proc Natl Acad Sci U S A,2005,102 (24):8585
  • 8Fouladi-Nashta AA,Jones CJ,Nijjar N et al.Characterization of the uterine phenotype during the peri-implantation period for LIF-null,MF1 strain mice.Developmental Biology,2005,281:1
  • 9Chen JR,Cheng JG,Shatzer T et al.Leukemia inhibitory factor can substitute for nidatory estrogen and is essential to inducing a receptive uterus for implantation but is not essential for subsequent embryogenesis.Endocrinology,2000,141:4365
  • 10Fouladi-Nashta AA,Andreu CV,Nijjar N et al.Role of leukemia inhibitor factor (LIF) in decidualisation of murine uterine stromal cells in vitro.Journal of Endocrinology,2004,181:477

共引文献8

同被引文献25

  • 1Ishihara N, Matsuo H, Mttrakoshi t1, et al. Increased apopt~)- sis in the syncytiotrophoblas! in human term placentas compli- cated by either preeclampsia or intrauterine growth retardation [J]. AmJ Obstet Gynecl, 2002, 186 (l): 158-166.
  • 2Longtine MS, Chen l/, Odibo AO, et Gl. Villous trophoblast apoptosis is elevated and restricted to cytotrophoblasts in preg- nancies complicated by preeelampsia, JUGR, or preeclampsiawith IUGR [J]. Placenta, 2012, 33 (5) : 352-359.
  • 3Grimson A, O'connor S, Newman CL, et al. SMG-1 is a phosphatidylinositol kinase-related protein kinase required for nonsense-mediated mRNA Decay in Caenorhabditis elegans [J]. Mol Cell Biol, 2004, 24 (17) : 7483-7490.
  • 4Du Y, Lu F, Li P, et al. SMG1 acts as a novel potential tumor suppressor with epigenetic inactivation in acute myeloid leuke- mia (J~. Int J Mol Sci, 2014, 15 (9) : 17065-17076.
  • 5Mcilwain DR, Pan Q, Reilly PT, et al. Smgl is required for embryogenesis and regulates diverse genes via alternative spli- cing coupled to nonsense-mediated mRNA decay [ J 1. Proc Natl Acad Sci U S A, 2010, 107 (27) : 12186-12191.
  • 6English FA, Kenny LC, Mc Carthy FP. Risk factors and effec- tive management of preeclampsia ~ J 1. Integr Blood Press Con- trol, 2015, 8: 7-12.
  • 7A1-Jameil N, Aziz Khan F, Fareed Khan M, et al. A brief o- verview of preeclampsia [J]. J Clin Med Res, 2014, 6 (1): 1-7.
  • 8Liao TL, Chen SC, Tzeng CR, et al. TCDD Induces the hy- poxia-inducible factor (HIF) -lct regulatory pathway in hu- man trophoblastic JAR cells ~ J~. Int J Mol Sci, 2014, 15(10) : 17733-17750.
  • 9Gubanova E, Issaeva N, Gokturk C, et al. SMG-1 suppresses CDK2 and tumor growth by egulating both the p53 and Cdc25A signaling pathways ( J 1. Cell Cycle, 2013, 12 ( 24 ) : 3770-3780.
  • 10Du W, Hong J, Wang YC, et al. Inhibition of JAK2/STAT3 signalling induces eolorectal cancer cell apoptosis via mito- chondrial pathway ~ J 1- J Cell Mol Med, 2012, 16 ( 8 ) : 1878-1888.

引证文献2

二级引证文献5

中国妇幼保健
2013年 第34期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部