白及药材HPLC指纹图谱研究

Study on HPLC Fingerprints of Bletilla Striata

目的:建立白及药材化学成分的HPLC指纹图谱,为白及药材的质量控制提供科学依据。方法:采用HPLC法,以Waters SunFire C_(18)(250 mm×4.6 mm,5μm)为色谱柱,Diamonsil C_(18)保护柱,以乙腈和水为流动相,梯度洗脱,流速为1.0 ml·min^(-1),检测波长为270 nm,柱温为30℃。结果:15批白及药材化学成分特征图谱共检出9个分离度良好的共有峰。白及药材9个特征峰进行聚类分析,白及药材产地与特征图谱相似度差异不大。样品中参照物1,4-二[4-(葡萄糖氧)苄基]-2-异丁基苹果酸酯(militarine)的含量有明显差别。结论:该法简便、快速,可作为白及的质量控制的有力手段。

Objective： To establish the HPLC fingerprints of chemical constituents in Bletilla striata to provide the basis for the quality control of Bletilla striata. Method： The chromatographic separation was performed on a Waters SunFire Cls （250 mm x 4.6 mm, 5 txm） column with a Diamonsil Cls guard column, and the mobile phase consisted of acetonitrile-water with gradient elution. The flow rate was 1.0 ml ~ min-~ , the detection wavelength was 270 nm, and the column temperature was 30~C. Result： There were 9 common peaks in the HPLC fingerprints of 15 batches ofBletilla striata, and the 9 characteristic peaks showed little change in Bletilla striata from different habitats. There was significant difference in the militarine content among the samples. Conclusion： The HPLC fingerprints method is simple, rapid and feasible in the quality control of Bletilla striata.