头孢唑兰对临床分离菌株体外抗菌作用评价

Antibacterial Effect of Cefozopran on Clinical Isolates in vitro

目的:评价头孢唑兰对临床分离菌株的体外抗菌作用,为头孢唑兰的临床使用提供参考依据。方法:用浊度法测定头孢唑兰对临床分离的无乳链球菌、甲氧西林敏感的金黄色葡萄球菌(MSSA)、甲氧西林敏感表皮葡萄球菌(MSSE)、肺炎链球菌、粪肠球菌、化脓性链球菌、大肠埃希菌、肺炎克雷伯菌、流感嗜血杆菌、阴沟肠杆菌、奇异变形杆菌、铜绿假单胞菌、产气肠杆菌等的最小抑菌浓度(MIC)和最小杀菌浓度(MBC),平行试验3次,观察头孢唑兰培养液的的浊度。结果:头孢唑兰对化脓性链球菌、肺炎链球菌、无乳链球菌、MSSE、MSSA、粪肠球菌6种临床分离G^+菌株的MIC分别为0.064,0.125,0.125,0.5,2,8μg·ml^(-1),MBC分别为0.125,0.25,0.25,1,4,16μg·ml^(-1);对肺炎克雷伯菌、大肠埃希菌、奇异变形杆菌、流感嗜血杆菌、产气肠杆菌、铜绿假单胞菌、阴沟肠杆菌7种临床分离G^-菌株的MIC分别为0.125,0.125,0.25,1,1,8,32μg·ml^(-1),MBC分别为0.25,0.25,0.5,2,2,16,64μg·ml^(-1)。结论:头孢唑兰具有较强的抑菌及杀菌作用。

Objective ： To evaluate the antibacterial effect of cefozopran on clinical isolates in vitro to provide the reference for the clinical use. Method ： The minimum inhibitory concentration （MIC） and minimum bactericidal concentration （MBC） of cefozopran a- gainst Streptococcus agalactiae, methicillin-sensitive Staphylococcus aureus （MSSA）, methicillin-sensitive Staphylococcus epidermidis （ MSSE ）, Streptococcus pneumoniae, Enterococcus faecalis, Streptococcus pyogenes, Escherichia coli, Klebsiella pneumoniae bacteria, Haemophilus influenzae bacteria, Enterobacter cloacae, Proteus mirabilis, Pseudomonas aeruginosa, and Enterobacter aerogenes were de- tected by turbidimetry. Result： MIC and MBC of cefozopran against the above clinical isolates in vitro were 0. 064, 0. 125, 0. 125, 0.5, 2, 8, 0. 125, 0. 125, 0.25, 1, 1, 8 and ml-j, and0. 125,0.25, 0.25, 1,4, 16, 0.25,0.25,0.5,2, 2, 16 and64 g ml-1, respectively. Conclusion： Cefozopran has strong bacteriostatic and bactericidal effects.