摘要
目的:测定鄂西北地区白及块茎中总酚的含量。方法:以没食子酸为对照品,采用Folin-酚比色法,运用可见-紫外分光光度法测定不同产地白及块茎中总酚的含量。结果:采用Folin-酚比色法测定白及中总酚含量,准确度好,精密度高。没食子酸对照品在0.005 3~0.063 6 mg·ml^(-1)的范围内线性良好(r=0.999 1),平均回收率为98.8%,RSD=1.55%(n=6)。结论:16种不同产地的白及药材中总酚含量差异较大,其中保康城关,河南淅川县荆紫关镇,及郧县鲍峡的白及药材含量较高,大于10.0%,产于郧县城关的白及药材含量较低,小于2.0%。白及产地不同,总酚含量相差较大,可作为控制白及质量的一项指标。
Objective： To determine total polyphenol in the tubers of Bletillae Striatae from northwest area of Hubei province. Method ： The content of total polyphenol in the tubers of BletiUae Striatae was detected by Folinphenol colorimetry using gallic acid as the reference and UV spectrophotometry. Result： There was a good linearity within the range of 0.005 3-0.063 6 mg ~ ml- l for gallic acid（r =0. 999 1 ）with the average recovery of 98.8% （RSD = 1.55% ,n =6）. Conclusion： There are significant differences in the to- tal polyphenol content from 16 areas. Among them, the total polyphenol content of BletiUae Striatae fl＇om Baokang Chengguan, Henan Xichuan Jingziguan, and Yunxian Baoxia is above 10.0% , while that of Bletillae Striatae from Zhushan and Yunxian Chengguan is lower than 2.0%. The content of total polyphenol in the tubers of BletiUae Striatae is various from different areas, which can be used as an index for the quality control of Bletillae Striatae.
出处
《中国药师》
CAS
2013年第11期1679-1682,共4页
China Pharmacist
关键词
白及
块茎
总酚含量
紫外分光光度法
Bletillae Striatae
Tuber
Total polyphenol content
UV spectrophotometry
