移植间充质干细胞在颅脑创伤小鼠小脑的定植与创伤修复

Colonization and Treuma Repair of Transplantated Mesenchymal Stem Cell in Cerebellum of Mouse with Brain Injury

目的探讨移植间充质干细胞(mesenchymal stem cells,MSCs)在颅脑创伤小鼠小脑的定植及参与创伤修复情况。方法绿色荧光蛋白(green fluorescent protein,GFP)转基因小鼠MSCs的分离培养。制作小鼠颅脑创伤模型后随机分2组,移植组于小鼠颅脑损伤后尾静脉注射分离培养成功的GFP转基因小鼠MSCs;生理盐水组于小鼠脑损伤后尾静脉注射生理盐水。对照组为正常C57BL/6小鼠。免疫组织化学法检测GFP标记MSCs在小鼠小脑的定植情况,并对三组小鼠进行神经行为学和运动功能评分。结果分离培养的GFP标记的MSCs在荧光显微镜下GFP阳性表达。移植组免疫组织化学染色显示在小脑颗粒细胞层边缘和靠近颗粒细胞层部位有阳性的GFP标记MSCs定植。在各时相点,创伤模型小鼠神经行为学和运动功能评分都与对照组有统计学差异(P<0.01)。第14天移植组神经行为学和运动功能评分与对照组比较无统计学差异,在第8天、14天与生理盐水组有统计学差异(P<0.05)。结论移植的MSCs能通过血液系统到达小脑创伤部位定植,并参与小脑功能的恢复。

Objective To explore the colonization and injury repair participation of the transplanted mesenchymal stem cells （MSCs） in the cerebellum of mice with brain injury. Methods MSCs of green fluorescent protein （GFP） trans genie mice were isolated and cultured. Model mice with brain injury were established and randomly divided into transplan tation group and saline group. GFPlabeled MSCs and saline were injected in the caudal vein in transplantation group and saline group respectively. The control group were normal C57BL/6 mice. The expressions of GFPlabeled MSCs were de tected by immunohistochemistry. The neurological behavior and motor function score of the three groups of mice were e valuated. Results The isolated and cultured GFPlabeled MSCs were positively GFP expressed under the fluorescence mi croscope. Transplant 10 days later, immunohistochemical staining showed sitepositive GFPlabeled MSCs colonization in cerebellar granule cell layer edge and near the granule cell layer. At all the timepoints, the model mice with trauma had significantly different neurological behavior and motor function score compared with that of the normal control group （P〈0. 01）. , The tranplantation had no difference with the control group at the 14th day, and had significant difference with the control group at the 8th and 14thday （P〉0.05）. Conclusion The transplanted MSCs can clolne in the trauma site inthe cerebellum through the blood system and participate in the recovery of cerebellar function.