曲美他嗪对心力衰竭大鼠的抗炎、抗氧化应激作用

The anti-inflammatory and anti-oxidative stress of Trimetazidine(TMZ) in rats with heart failure

目的:探讨曲美他嗪对心力衰竭大鼠抗炎、抗氧化应激作用。方法:采用腹主动脉缩窄法制备SD大鼠心力衰竭模型,双抗体夹心ELISA法检测血浆CRP、IL-6和TNF-α的表达水平;real-time PCR定量分析超氧化物歧化酶(SOD)、血红蛋白氧合酶(HO-1)、谷胱甘肽过氧化物酶(GPX)表达;免疫组织化学法检测SOD;放射免疫法检测总抗氧化能力(T-AOC)。结果:模型组血浆C反应蛋白、IL-6和TNF-α水平升高,曲美他嗪治疗组C反应蛋白、IL-6和TNF-α水平下降(P<0.01或0.05);模型组SOD、GSH-Px、HO-1表达和T-AOC水平明显降低(P<0.01或0.05)。曲美他嗪治疗后,SOD、GPx、HO-1和T-AOC表达水平均有所升高,以SOD、T-AOC改善明显(P<0.05、P<0.01),高剂量组SOD表达甚至高过假手术组。结论:曲美他嗪能减少心衰大鼠模型炎症因子的表达,提高心肌氧化相关基因的表达,从而改善心功能。

Objective：To Explore The anti-inflammatory and anti-oxidative stress of Trimetazidine（TMZ） in rats with heart failure. Methods： Rat models with congestive heart failure were achieved by partially banding the abdominal aortic arteries. The expression levels of plasma CRP, IL-6 and of TNF-α were detected by Double-antibody sandwich ELISA. The expression of superoxide dismutase（SOD）, heme oxygenase（ HO-1） and glutathione peroxidase（GPX） were measured by Real-Time PCR. SOD was detected by immunohistochemistry. T-AOC was detected by Radioimmunoassay Method. Re-sults：Compared with the Sham operation group, the CRP, IL-6 and TNF-αwere increased in the heart failure models, and were significantly decreased in the TMZ treatment group（P〈0.01 or P〈0.05）. The SOD, GPX, HO-1 and T-AOC were sig-nificantly decreased in the heart failure models （P〈0.01 or P〈0.05） and increased in the TMZ treatment group,and SOD and T-AOC were especially increased （P〈0.05, P〈0.01）. The activity of SOD of the high dose was higher than in the sham operation group. Conclusion：Trimetazidine can reduce the expression of inflammatory factors in rat models with heart fail-ure, and improve the myocardial oxidation-related gene expression, thereby improving cardiac functions.