癌胚抗原mRNA、人中性粒细胞多肽1-3联合检测在结直肠癌诊断中的价值

Preliminary study of the diagnosis of colorectal cancer by single and combined detection with peripheral blood CEAmRNA and HNP1-3

目的:探讨外周血癌胚抗原信使核糖核酸(CEAmRNA)、中性粒细胞多肽1-3(HNP1-3)单一检测及联合检测在结直肠癌诊断中价值。方法:选取结直肠癌70例患者为观察组,另选良性疾病患者70例为对照组。分别采用Nested RT-PCR法检测CEAmRNA,ELISA法检测HNP1-3,观察两组患者CEAmRNA及HNP1-3的表达。结果:CEAmRNA对结直肠癌的诊断敏感度为52.86%,特异度为98.57%;HNP1-3对结直肠癌的诊断敏感度为60%,特异度为98.57%。两者联合检测时至少1项阳性敏感度为77.14%,特异度为97.14%,2项均阳性敏感度为42.85%,特异度为100%。CEAmRNA、HNP1-3的表达与淋巴结转移及Dukes分期相关。结论:在结直肠癌诊断中,外周血CEAmRNA和HNP1-3二者平行相关检测具有更高价值和诊断意义。

Objective： To explore the diagnosis of colorectal cancer by the single detection,parallel and series detection with peripheral blood CEAmRNA and HNP1-3. Methods： The expression of CEAmRNA was detected by using Nested RT-PCR in 70 patients with colorectal cancer （the experimental group） and in 70 normal subjects （the control group）. The expression of HNP1-3 was detected by using ELISA in both groups. Results：The sensitivity of CEAmRNA and HNP1-3 in the experimental group was 52.86% and 60%, respectively. The specificity of CEAmRNA and HNP1-3 in the experimental group was 98.57%. A higher sensitivity （77.14%）was shown when either of the CEAmRNA and HNP1-3 was positive. A higher specificity（100%）was found when both of the CEAmRNA and HNP1-3 were positive. The expressions of CEAmRNA and HNP1-3 were not significantly correlated with the patient’s sex, age, position of tumor or differentiation level of cancer cells, but with the clinical stage and lymph node metastasis. Conclusion：In the diagnosis of colorectal cancer, the single detection plays a certain role. But the parallel and series detections are more valuable and diagnostically significant.