抗松材线虫病马尾松离体培养植株再生试验

Plantlet regeneration of pine wilt disease-resistant Pinus massoniana in vitro

以萌发25 d的抗松材线虫病和非抗病马尾松无菌苗子叶/胚轴部分为外植体建立了植株再生体系。外植体接种于GD+4.0 mg/L 6-BA+0.2 mg/L NAA培养基上40 d可诱导子叶基部丛生芽形成。将芽连同外植体转移至DCR+0.2 mg/L NAA培养基上可促进芽的生长,活性炭促进了芽的进一步伸长,以伸长的芽为外植体,接种于1/2GD+3.0 mg/L 6-BA+0.2 mg/L NAA培养基上可实现丛生芽的大量增殖。伸长的芽接种于WPM+4 mg/L IBA+0.2 mg/L NAA培养基中28 d后,不定根发生率为33%。

A experiment on plantlet regeneration for pine wilt disease-resistant Pinus raassoniana was carried out by using cotyledon-hypocotyl obtained from 25 d old aseptically grown seedlings as explants. Cotyledon-hypocotyl explants were cul-tured on a GD medium containing 4 mg/L 6-BA （6-benzylaminopurine） and 0.2 mg/L NAA （cx-naphthylacetic acid） for 40 days to stimulate the formation of intercotyledonary axillary buds. The axillary buds grew vigorously after being trans- ferred to DCR medium containing 0.2 mg/L NAA. Axillary buds elongated rapidly on DCR medium containing 0.5 g/L ac-tivated charcoal. Shoot proliferation was achieved by cutting elongated shoots into stem segments and subculturing on GD medium containing 3 mg/L BA and 0. 2 mg/L NAA. Roots were formed when excised shoots planted on WPM medium supplemented with 4 mg/L IBA and 0.2 mg/L NAA for 4 weeks, and the rooting rate was 33%.