微生物制剂MP代谢产物的蛋白质组分析

Proteome analysis of metabolic products by microbial agents MP

为明确微生物制剂MP提高烟叶品质的原因,以该制剂的代谢产物作为研究材料,采用液相色谱串联质谱法(LC-MS/MS)分析了微生物制剂MP代谢产物的蛋白质组成分。结果共鉴定出35个非重复蛋白质,蛋白等电点几乎均在4-7范围内,分子量均大于20 kD。KEGG代谢通路分析显示,所鉴定的蛋白几乎都与代谢有关,其中参与氨基酸代谢、糖酵解、三羧酸循环、丙酮酸盐代谢的蛋白最多。其中,氨基酰组氨酸二肽酶、嗜热菌状金属蛋白酶、尿刊酸水合酶和组氨酸氨裂解酶能有效降低烟叶中蛋白质。

Proteome of MP metabolic productions was analyzed by LC-MS/MS to determine the mechanism of MP in increasing tobacco quality. 35 non-repeated proteins were identified whose isoelectric point fall within the scope of 4 to 7 and molecular weight are more than 20 kD. KEGG analysis indicated that those proteins were involved in metabolic pathways especially in amino acid metabolism, glycolysis, tricarboxylic acid cycle and pyruvate metabolism. Enzymes such as aminoacyl-histidine dipeptidase, thermolysin-like metalloprotease, HutU urocanate hydratase and histidine ammonia-lyase, function in protein degradation were vital for increasing tobacco quality after MP spraying.