生活方式干预与细胞免疫功能的相关性研究

A study of the association between life style intervention and cellular immune function

目的探讨健康生活模式对机体自然清除HPV病毒的价值。方法对HPV感染者随机分为干预生活方式实验组和对照组,分别对实验组和对照组进行宫颈分泌物T淋巴细胞及其亚群(CD3+、CD4+、CD8+、CD4+/CD8+)定量检测,半年后复查宫颈HPV病毒和宫颈分泌物T淋巴细胞及其亚群定量检测。结果实验组的HPV病毒清除显著高于对照组(P<0.01);实验组和对照组在干预治疗前CD3+、CD4+、CD8+、CD4+/CD8+组间均无显著性差异;实验组干预治疗后CD3+、CD4+、CD4+/CD8+较实验前显著升高(P<0.01),而CD8+显著下降(P<0.01);对照组在实验前后CD3+、CD4+、CD8+、CD4+/CD8+均无显著性差异(P>0.05)。结论对HPV感染者进行生活方式干预治疗,有助于患者免疫能力的改善和病毒的清除,降低宫颈癌病变和宫颈癌的发生。

Objective To investigate the effect of life style intervention on the ability of immune system to clear up HPV. Methods 258 patients with histologically confirmed HPV infection were recruited and randomly divided into intervention group and control group. Samples of cervical secretion were collected and total T lymphocytes and its subgroups and HPV were quantified using flowcytometry and real time PCR respectively. The measurement was repeated after 6 months. Results HPV clearance in the intervention group was significantly higher than the control group （P〈0.01）. No difference was identified between the two groups regarding the total and subgroups of T lymphocytes. Additionally, the treatment could significantly improve the counts of T lymphocytes in the intervention group （P〈0.01）, while such effect was not observed in the control group. Conclusion Life-style intervention could improve the immune function and help to clear up the viruses in the HPV-infected patients.