胆腑舒胶囊对实验性胆囊结石胆汁卵磷脂、总胆汁酸、粘蛋白及血清脂代谢的影响

The effect of Danfushu capsule on bile lecithin,total bile acid,mucin and serum lipid metabolism in experimental gallstone rabbits

目的 :观察胆腑舒胶囊对家兔实验性胆固醇结石的溶石作用及机制。方法 :血清脂代谢指标在全自动生化仪上完成。胆汁中总胆固醇 (TC)、总胆汁酸分别用硫磷铁法、磷钼酸比色法测定。胆汁中卵磷脂、粘蛋白分别运用硝化磷钼酸氨萘磺酸法、磷钨酸沉淀酚试剂法测定。结果 :胆腑舒胶囊可有效地降低血清 TC、甘油三酯 (TG)、低密度脂蛋白胆固醇 (L DL C)及胆汁中胆固醇、粘蛋白浓度 ,提高胆汁中总胆汁酸、卵磷脂含量。结论 :胆腑舒胶囊能较好地溶、排胆囊胆固醇结石 ,其机制与调节脂质代谢。

Objective:To observe the stonelysis effect of Danfushu capsule(胆腑舒胶囊) on cholesterol stone in rabbit model and its mechanism.Methods:The parameters of serum lipid metabolism were detected by full automatic biochemical instrument.The total cholesterol(TC) and total bile acid in bile gall were measured by nemafos method and phosphomolybdic acid colorimetry respectively,and the bile lecithin and mucin were done by nitrificationphosphomolybdic acidnaphthionic acid and phosphotungstic acid precipitationphenol reagent method respectively.Results:Danfushu capsule could obviously reduce the concentrations of serum total cholesterol(TC),triglycerides(TG),lowdensity lipoprotein cholesterol(LDLC) ,bile cholesterol,and mucin,meanwhile increase the contents of total bile acid and lecithin in bile gall.Conclusions:Danfushu capsule is good at lytic and eliminating effects on cholesterol gallstone.The mechanisms are related to the regulation of lipid metabolism,improvement of pathogenetic bile,and gallbladder.