肾衰宁灌肠液对体外培养的人肾小球系膜细胞增殖及产生纤维连接蛋白的影响

Effect of Shenshuaining on proliferation and fibronectin production of human mesangial cells cultured in vitro

目的 :观察肾衰宁 (SSN)灌肠液对体外培养的人肾小球系膜细胞增殖及产生纤维连接蛋白 (FN)的影响 ,以便从细胞生物学水平探讨肾衰宁防治慢性肾功能衰竭 (CRF)的作用机制。方法 :采用 4甲基偶氮唑盐(MTT)比色法和双抗夹心酶联免疫吸附 (EL ISA)法 ,观察 SSN大鼠血清对体外培养的人肾小球系膜细胞增殖和 FN生成的影响。结果 :SSN大鼠血清可抑制体外培养的人肾小球系膜细胞增殖和产生 FN,其抑制程度与药物浓度有一定的量效关系 :即随药物浓度的增加 ,对系膜细胞增殖和产生 FN的抑制率也相应增加。结论 :SSN对体外培养的人肾小球系膜细胞增殖和产生 FN有明显的抑制作用 ;SSN灌肠液抑制肾小球系膜细胞增殖和 FN产生是预防肾小球硬化发生发展的重要机制之一。

Objective:To observe the effects of traditional Chinese herbs Shenshuaining (SSN,肾衰宁) eultured in vitro,in order to investigate the mechanisms of SSN on preventing and treating chronic renal failure(CRF) in cytobiological level.Methods:By means of MTT colorimetry and enzymelinked immunosorbent assay the effect of serum of rat using SSN on proliferation and FN production of huMsC cultured in vitro was observed.Results:The serum of rat using SSN was able to inhibit the proliferation and FN production of huMsC cultured in vitro and the volumeefficacy relationship was existed.As the SSN concentration increased the inhibiting rate increased correspondingly.Conclusions:The SSN enema liquor possesses obviously inhibiting action on proliferation and FN production of huMsC cultured in vitro so that this is one of the important mechanisms on preventing the occurance and progress of glomerulosclerosis.