脑胶质瘤p15基因缺失及5′CPG岛甲基化研究

Deletion and 5′CPG Island Methylation of p15 Gene in Brain Glioma

目的 探讨p15基因变异及其与脑胶质瘤的发生、恶性进展的关系。方法 利用PCR和PCR -based甲基化检测技术检测了5 6例脑胶质瘤中p15基因外显子 1缺失及 5′CPG岛甲基化情况。结果 43例高级别的脑胶质瘤中 ,14例发生了p15基因缺失( 3 2 6% ) ,而 13例低级别的脑胶质瘤中无一例发生p15基因缺失 ,差异具有显著性 (P <0 0 5 )。 1例低级别的脑胶质瘤、3例高级别的脑胶质瘤发生了p15基因 5′CPG岛甲基化。结论 p15基因异常可能参与脑胶质瘤的发生、恶性进展。基因纯合缺失是脑胶质瘤中p15基因失活的主要机制。

Objective To investigate the abnormality of p15 gene in brain glioma and its correlation with the occurrence or malignant progression of brain glioma.Methods Deletion and 5′CPG island methylation of p15 gene were detected by means of PCR and PCR-based methylation in 56 cases of brain glioma.Results Of the 43 cases of high grade glioma,14 were found to have homozygous deletion of p15 E1,while none of the 13 cases of low grade glioma was found to have deletion of p15 E1( P <0 05).Methylation of 5′CPG island of p15 gene was found only in four cases of glioma.Conclusions Abnormality of p15 gene may be involved in the occurrence and malignant progression of brain glioma.Homozygous deletion of gene is the major mechanism of inactivation for p15 gene in brain glioma.