HIF-1α和VEGF在大肠癌中的表达及其相关性研究

Expressions and Correlation of HIF-1α and VEGF in Colorectal Cancer

[目的]探讨HIF-1α和VEGF在大肠癌浸润、转移过程中的表达及其相互作用机制。[方法]采用免疫组化SP法检测90例大肠癌组织及其相应癌旁组织中HIF-1α和VEGF的表达。[结果]癌旁组织中HIF-1α的表达均为阴性,大肠癌组织中的阳性表达率为73.33%(66/90),显著高于癌旁组织,差异有统计学意义(P<0.05)。HIF-1α在大肠癌中阳性表达率与临床分期、浸润深度和淋巴结转移有关(P<0.05),与大肠癌分化程度无关(P>0.05)。癌旁组织中VEGF的表达均为阴性,在大肠癌组织中的阳性表达率为70.00%(63/90),显著高于癌旁组织,差异有统计学意义(P<0.05)。VEGF在大肠癌中阳性表达率与临床分期、浸润深度和淋巴结转移有关(P<0.05),与大肠癌分化程度无关(P>0.05)。大肠癌组织中HIF-1α和VEGF蛋白表达具有正相关性(r=0.592,P<0.05)。[结论]HIF-1α与VEGF的表达率均随着大肠癌浸润深度、淋巴结转移和临床分期的发展而增高,共同参与了大肠癌的发生、发展,可作为大肠癌浸润和转移的重要判定指标。HIF-1α可能通过上调VEGF的蛋白表达,促进大肠癌血管生成而促进大肠癌的转移,抑制HIF-1α的表达可能是治疗大肠癌的新手段。

To investigate the expression of HIF-α and VEGF in invasion and metastasis of colorectal cancer and their interaction mechanism.[Methods]The expressions of HIF-1 and VEGF were detected by SP immunohistochemistry method in cancer and cancer adjacent tissues of 90 cases with colorectal cancer. [Results]The expression of HIF-1α in cancer adjacent tissues was negative,while the positive expression rate was 73.33%（66/90） in cancer tissues,with significant difference between them（P〈0.05）. The expression of HIF-1α was significantly associated with the depth of invasion,lymph node metastasis and Dukes stage（P〈0.05）,but not associated with the grade of differentiation（P〈0.05）. The expression of VEGF in cancer adjacent tissues was negative,while the positive expression rate was70.00%（63/90） in cancer tissues,with significant difference between them（P〈0.05）. The expression of VEGF was significantly associated with the depth of invasion,lymph node metastasis,and Dukes stage（P〈0.05）,but not associated with the grade of differentiation（P〈0.05）. The expression of HIF-1α and VEGF was positively correlated（r=0.592,P〈0.05）. [Conclusion]The expression of HIF-1α and VEGF increases with the increasing lymph node metastasis,cancer invasion and clinical staging. They both take part in the carcinogenesis of colorectal cancer,and might be important indicators for the diagnosis of invasion and metastasis of colorectal cancer. HIF-1α might promote tumor angiogenesis and invasion by upregulating the expression of VEGF protein. Therefore,inhibition the expression of HIF-1α might be a new method for the treatment of colorectal cancer.