人淋巴瘤Raji/DEX耐药细胞株的建立及其耐药机制

Establishment of human lymphoma drug-resistant cell lines Raji /DEX and their mechanism of drug resistance

目的建立人淋巴瘤耐药细胞株Raji/地塞米松(DEX)并对其耐药机制进行探讨。方法分别采用噻唑蓝还原法(MTT)确定Raji细胞的半数抑制浓度(IC50)值,DEX浓度递增和持续作用的方法体外诱导Raji细胞的耐药性并检测其耐药指数,Western blot法检测人核转录因子p65(NF-κBp65)蛋白。结果历时3个月后建成的细胞株Raji/DEX增殖速度减慢,体积变小,且对DEX低度耐药,耐药指数为1.7(P<0.05);Raji/DEX的NF-κBp65蛋白表达显著高于Raji亲本细胞(P<0.05)。结论成功建立Raji/DEX耐药细胞株。NF-κBp65蛋白表达增强可能是淋巴瘤细胞产生获得性耐药的主要机制之一。

Objective To establish human lyinphoma drug-resistant cell lines Raji/ DEX and investigate their mechanism of drug resistance. Methods ICs0 of Raji cells was determined by MTT. Drug resistance of Raji cells was induced in vitro by increase in concentration and sustained action of Dexamethasone, and its drug resistance index was calculated. NF-KBp65 was detected by Western ＇blot. Results When compared to Raji parent cells, Raji/DEX established after 3 months showed slower rate of prolifera- tion, smaller size, and lower resistance to Dexamethasone with a drug resistance index of 1.7 （ P 〈 0.05 ）. NF-KBp65 in Raji/DEX was increased （ P 〈 0.05 ）. Conclusion Human lymphoma drug-resistant cell lines Raji/DEX is successfully established. The increased NF-KBp65 may be one of mechanisms underlying acquired drug resistance of lymphoma cells.