小儿特发性血小板减少性紫癜外周血T、B淋巴细胞观察

The observation of T and B lymphocyte in peripheral of infantile idiopathic thrombocytopenic purpura

目的：检测ＩＴＰ患儿Ｔ淋巴细胞亚群及Ｂ细胞的比值变化。方法：采用荧光免疫法用淋巴细胞分离液配制单个细胞，温育后加入抗体，计算阳性百分率。结果：患儿组 Ｔ３、Ｔ４与健康组差异不显著，ＴＳ及 Ｂ细胞患儿组与健康组差异有显著性。结论：ＩＴＰ患儿可出现细胞免疫及体液免疫功能紊乱。

Objective: To detect the ratio changes of subgroup T lymphocyte and B lymphocyte in infants with ITP. Methods: Manufacturing single nucleus cell with lymphocyte, separate liquid, adding antibody after thermal cultivation and calculating the percentage of positive cells using inmuno-fluorescence method. Results: There are no statistie differences between the ITP patients' T3、T4 and the healthy contral's there are statistie differences between the ITP patients' T8、B groups and the healthy contral's.Conclusion: The patients of infantile ITP may have humoral and cellular immunity factional disorder.