布鲁菌保护性抗原基因植物表达载体的构建

Construction of Plant Expression Vectors Containing Brucella Protective Antigen Genes

根据genbank序列,参照植物偏性密码子,设计合成适合植物表达的布鲁菌rplL基因和omp31基因。将携带植物表达优化序列的rplL基因片段与pBI-121载体相连接构建重组植物表达载体pBI121-rplL;将omp31基因与相应酶切并携带植物表达特征序列的pMD19-T6载体连接获得重组质粒,之后酶切获得携带植物表达优化序列的omp31基因片段,将其克隆入pBI-121载体,从而构建出带有不同目的片段的重组植物表达载体,为下一步检测基因在植物中的表达奠定基础。

Based on the genbank sequence and plant expression codons, the Brucella rplL gene and omp31 gene were designed and synthesized which might be suitabale for plant expression. Rill gene with plant expression elements was digested and introduced to plant expression vector pBI121 directly to construct a recombinant plant ex- pression vector pBI121-rplL. Omp31 gene was firstly connected with the corresponding digested pMD19-T6 which carried the specific sequences suitable for plant expression. Then the target gene with plant expression elements was subcloned into pBI-121. Thus different recombinant plant expression vectors with the protective antigen genes of Brucella were successfully constructed. It has laid the foundation for the furthur study on the detection of the ex- pression of certain genes in plants.