摘要
目的探讨普罗布考(probucol,PRB)对高糖培养的大鼠肾小球系膜细胞(rat mesangial cells,RMCs)增殖及转化生长因子-β1(TGF-β1)的影响。方法①将RMCs分为正常对照组(葡萄糖5.5 mmol·L-1)、高糖组(葡萄糖30.0 mmol·L-1)、高糖+不同浓度PRB组(葡萄糖30.0 mmol·L-1+10、20、50μmol·L-1PRB),MTT法检测培养24、48、72 h后细胞的增殖情况,ELISA法检测培养24 h后TGF-β1分泌量。②将RMCs分为正常对照组(葡萄糖5.5 mmol·L-1)、渗透浓度对照组(5.5 mmol·L-1葡萄糖+24.5 mmol·L-1甘露醇)、高糖组(葡萄糖30.0 mmol·L-1)、高糖+20μmol·L-1PRB组,ELISA法检测培养12、24、48、72 h后TGF-β1分泌量。结果①培养24 h后,高糖刺激RMCs增殖,PRB呈时间依赖性和剂量依赖性地抑制RMCs的增殖(P<0.05)。②高糖刺激24 h后,TGF-β1分泌量增多,PRB能抑制高糖诱导的TGF-β1分泌(P<0.05)。结论 PRB可能通过抑制肾小球系膜细胞TGF-β1的分泌,抑制高糖条件下大鼠肾小球系膜细胞的增殖,发挥肾脏保护作用。
Objective To explore the effect of probucol （PRB） on the expression of transforming growth factor β1 （TGF-β1） and proliferation in rat mesangial cells （RMCs） induced by high glucose. Methods ① Cultured RMCs were divided into a normal contrast group （5.5 mmol ·L^-1 glucose）, a high glucose group （30.0 mmol·L^-1 glucose）, and a high glucose plus PRB group （30.0 mmol ·L^-1 glucose ＋ 10 or 20 or 50 μmol·L^-1 PRB）, and cultured for 24, 48 and 72 h. The proliferation of RMCs was detected by micro-tetrazolium salt colorimetric enzyme reaction test （MTT）. The secretion of TGF-β1 was measured by ELISA. ② Cultured RMCs were divided into a normal contrast group （5.5 mmol ·L^-1 glucose）, an iso-osmolar control group （5.5 mmol ·L^-1 glucose ＋ 24.5 mmol ·L^-1 mannitol）, a high glucose group （30.0 mmol ·L^-1 glucose）, and a high glucose ＋ 20 μmol ·L^-1 PRB group. The secretion of TGF-β1 was measured by ELISA after cluturing for 12, 24, 48 and 72 h. Results ① High glucose increased the proliferation of RMCs at 24 h, and PRB inhibited the proliferation in both dose-dependent and time-dependent manner （P 〈 0.05）. ③High glucose increased the secretion of TGF-β1 at 24 h, and PRB inhibited the secretion of TGF-β1（P 〈 0.05）. Conclusion PRB can inhibit the proliferation induced by high glucose in RMCs by decreasing the secretion of TGF-β1 and play a role in kidney protection.
出处
《中南药学》
CAS
2013年第10期729-731,共3页
Central South Pharmacy
