香蜂花中迷迭香酸的提取工艺优选

Optimization of Extraction Technology for Rosmarinic Acid in Melissa officinalis L.

目的： 优选香蜂花中迷迭香酸的提取工艺。 方法： 以迷迭香酸含量为指标，通过单因素试验考察提取溶剂、提取方式、提取时间及溶剂用量对工艺的影响，采用正交试验考察提取次数、提取时间和加醇量对迷迭香酸含量的影响，优选香蜂花的提取工艺。采用HPLC测定迷迭香酸含量，色谱条件为Hanbon Megres C18色谱柱（4.6 mm×250 mm，5 μm），流动相甲酸-乙腈-水（0.5∶20∶80，A）与甲酸-甲醇-乙腈（0.5∶40∶60，B）梯度洗脱（0~25 min，0%~45%B;25~30 min，45%~100%B），检测波长330 nm，进样量20 μL。 结果： 最佳提取工艺为加10倍量70%乙醇回流提取3次，每次1 h;迷迭香酸平均提取量达25.27 mg·g-1。 结论： 优选的工艺稳定可行，为香蜂花的资源开发提供参考。

Objective： To optimize extraction technology of rosmarinic acid in Melissa officinalis. Method： With the content of rosmarinic acid as index,based on single factor tests,effects of extraction time,extraction times and the amount of ethanol on extraction technology were investigated by orthogonal test.HPLC was employed to determine the content of rosmarinic acid and the chromatographic conditions were as follows：Hanbon Megres C18 column（4.6 mm250 mm,5 μm）,mobile phase of formic acid-acetonitrile-water（0.5：20：80,A） and formic acid-methanol-acetonitrile（0.5：40：60,B） with gradient elution program（0-25 min,0%-45% B;25-30 min,45%-100% B）,detection wavelength 330 nm,injection volume 20 μL. Result： Optimal extraction technology was as following：extracted 3 times with 10 times the amount of 70% ethanol,1 h each time;Yield of rosmarinic acid was up to 25.27 mg·g-1. Conclusion： Optimized technology was stable and feasible to provide a reference for resource development of M. officinalis.