Plackett-Burman联用正交设计优选长叶胡颓子总三萜酸纯化工艺

Optimization of Purification Technology for Total Triterpenoid Acids from Elaeagnus bockii Diels by Plackett-Burman Combined with Orthogonal Test

目的:优选长叶胡颓子总三萜酸的大孔吸附树脂纯化工艺。方法:采用UV测定总三萜酸含量。通过动态吸附-洗脱试验筛选大孔树脂型号;以总三萜酸含量为指标,通过单因素试验筛选纯化工艺的影响因素,Plackett-Burman试验筛选主要影响因素。以总三萜酸含量和浸膏得率的综合评分为指标,选取乙醇体积分数、洗脱剂用量、醇碱比为考察因素,通过正交试验优选长叶胡颓子总三萜酸的富集工艺。结果:长叶胡颓子总三萜酸的最佳纯化工艺为洗脱剂用量4 BV,醇碱比1∶5,乙醇体积分数80%,洗脱流速3 mL·min-1;总三萜酸质量分数达75%,浸膏收率达22%。结论:利用Plackett-Burman试验设计联用正交试验优选的纯化工艺稳定、简便易行,为长叶胡颓子三萜酸的开发利用提供实验依据。

Objective： To optimize purification process of total triterpenoid acids from Elaeagnus bockii by macroporous resin. Method： The content of total triterpenoid acids was determined by UV.Type of macroporous resin was screened by dynamic adsorption-elution test;With the content of total triterpenoid acids as index,influence factors were selected by single factor tests,main factors were investigated by Plackett-Burman.Taking composite score of the content of total triterpenoid acids and extract yield as index,L9（34） orthogonal test was applied to optimize purification process of total triterpenoid acids with ethanol concentration,alcohol amount,ratio of ethanol and 1% sodium hydroxide as factors. Result： Optimum purification process conditions were as follows：eluent amount 4 BV,ratio of 80% ethanol and 1% NaOH （1：5）,flow rate 3 mL min-1;The mass fraction of total triterpenoid acids was 75%,extract yield was more than 22%. Conclusion： This optimized process was simple and stable to provide experimental basis development and utilization of total triterpenoid acids from E. bockii with high precision and predictability.