星点设计-效应面法优化首乌藤微波提取工艺

Optimization of Microwave Extraction Technology for Caulis Polygoni Multiflori by Central Composite Design-Response Surface Methodology

目的:优选首乌藤改善睡眠有效部位的微波辅助提取工艺。方法:采用单因素试验考察微波功率、提取时间、乙醇体积分数、溶剂用量、提取温度等对首乌藤总黄酮和总蒽醌含量的影响。在单因素试验基础上,选取微波功率、提取时间、乙醇体积分数为考察因素,每个因素设置5个水平,以总黄酮、总蒽醌含量的总评"归一值"为评价指标,利用星点设计-效应面法优选微波提取工艺。结果:首乌藤改善睡眠有效部位的最佳微波提取工艺为微波功率585 W,提取数1次,加10倍量82%乙醇于60℃提取11 min。结论:优选的微波辅助提取工艺简便易行,预测性良好,为首乌藤后续的剂型开发和工业化生产提供实验依据。

Objective： To optimize microwave extraction process of effective parts in Caulis Polygoni Multiflori for improving sleep. Method： Single-factor tests were adopted to investigate effects of extraction time,ethanol concentration,microwave power,solvent amount and extraction temperature on contents of total flavonoids and total anthraquinones in Caulis Polygoni Multiflori.Based on single factor tests,taking extraction time,ethanol concentration and microwave power as factors,each factors was set five levels,with the ＂Overall Desirability＂ of contents of total flavonoids and total anthraquinones as evaluation indicator,then extraction process of effective parts in Caulis Polygoni Multiflori for improving sleep was optimized by central composite design-response surface methodology. Result： Optimum microwave extraction conditions were as follows：microwave power 585 W,extracted 11 min with 10 times the amount of 82% ethanol at 60℃,extracted 1 time. Conclusion： Optimized extraction technology was simple and feasible with a good predictability,it could provide experimental evidence for formulations development and industrial production of Caulis Polygoni Multiflori.