丹参内生真菌与其有效成分的相关性分析

Correlation between Active Components and Endophytic fungi in Salvia miltiorrhiza

目的:探讨不同产地丹参根中内生真菌的种群与丹参主要有效成分的相关性。方法:采用组织块分离法分离丹参根中的内生真菌,采用插片法和点植法分类鉴定分离的菌株;采用HPLC测定丹参酮ⅡA、隐丹参酮、丹酚酸B与丹参素的含量。结果:从全国25个产地丹参的根中共分离得到373株内生真菌,经显微形态观察,鉴定为1纲3目36属;不同产地丹参内生真菌与其有效成分具有相关性。结论:不同产地丹参的内生真菌在数量、分布、种群以及组成上存在差异,丹参内生真菌的种群结构与其质量的形成有关。

Objective： To study the correlation between active components and Endophytic fungal community in Salvia mihiorrhiza from different habitats. Method： Tissue separation is applied to separate the E. fungi in roots of S. mihiorrhiza. The fungi are classified and identified by insert training methods and point-sik methods. The content of tanshinoneⅡA,cryptotanshinone,salvianolic acid B and danshensu are determined by HPLC. Result： E. fungal are abundance in species and quantity,which are generally found in the roots of S. mihiorrhiza from different habitats,are identified into 3 orders,4 families and 36 genera.The correlation between active components and E. fungi in S. mihiorrhiza was revealed. Conclusion： Endophytic fungi in S. mihiorrhiza from different habitats in terms of quantity,distribution,populati on and its components are different,and the correlation between E. fungal and their quality formation is revealed.