茜草药材的HPLC指纹图谱研究

Quality Control of Madder Based on HPLC Fingerprint

目的:建立河南不同产地茜草药材的HPLC指纹图谱分析方法,为茜草药材的质量评价提供参考依据。方法:采用高效液相色谱法,Phenomenex ODS C18柱(4.60 mm×250 mm,4μm),甲醇-0.2%磷酸水梯度洗脱,检测波长275 nm,流速0.8 mL·min-1,柱温25℃。结果:以大叶茜草素为参照峰,确定11个共有峰,测定了14批茜草药材HPLC指纹图谱与对照图谱的相似度。结论:所建立的方法可用于茜草药材指纹图谱测定,并可对其今后规范药用资源及质量评价提供科学依据。

Objective： To establish the HPLC fingerprint analysis methods for Madder and to provide the reference for quality evaluation of Madder. Method： Phenomenex ODS C18（4.60 mm250 mm,4 μm）column was used and the methanol-0.2% H3PO4 water was chosen as the mobile phase in a gradient mode. The column temperature was at 25℃ and the detection wavelength was at 275 nm.The detection time was 80 min and the flow rate was 0.8 mL min-1. Result： Taking mollugin as the reference peak,eleven common peaks were selected as the fingerprint peaks of Madder. Conclusion： This method is simple and accurate with a good reproducibility. It provides a reference standard for the quality control of Madder.