离子色谱法测定栀子中Cl^-,Br^-,NO_3^-,SO_4^(2-),PO_4^(3-)5种阴离子的含量

Determination the Content of Cl^-,Br^-,NO_3^-,SO_4^(2-),PO_4^(3-) in Chinese Herbal of Gardenia by Ion Chromatography

目的:建立一种同时测定中药栀子药材中Cl-,Br-,NO-3,SO2-4,PO3-4阴离子的含量测定方法。方法:采用离子色谱法,IonPac AS11-HC阴离子色谱柱,氢氧化钾梯度淋洗,电导检测栀子中5种无机阴离子。结果:Cl-,Br-,NO-3,SO2-4,PO3-4的线性范围良好,加样回收率介于97%~102%,栀子中Cl-,Br-,NO-3,SO2-4,PO3-4重复性良好,含量分别为0.084%,0.063%,0.070%,0.30%,0.12%。结论:离子色谱法可以同时检测栀子中5种阴离子,灵敏度高,结果准确可行,为栀子的质量控制提供了一种简便快速的方法。

Objective： To establish a simultaneous determination of Cl-,Br-,NO3-,SO42-,PO43- content of gardenia by ion chromatography. Method： The ICS-900 ion chromatography and IonPac AS11-HC inorganic anion exchange chromatography column,KOH gradient elution and conductivity detection were adapted to assay the five kinds of inorganic anion. Result： Cl-,Br-,NO3-,SO42-,PO43- were good in linearity. Samples recovery were between 97%-102%. The content of Cl-,Br-,NO3-,SO42-,PO43- were 0.084%,0.063%,0.070%,0.30% and 0.12%. Conclusion： The method is sensitive,accurate and operational,provided a simple and quickly method to assay the content of Cl-,Br-,NO3-,SO42-,PO43- in gardenia.