独一味不同提取物中总环烯醚萜苷、总黄酮和总苯乙醇苷的测定

Mass Fraction Determination of Iridoid Glycosides,Flavonoids and Phenylethanol Glycosides from Different Extractive of Extract of Lamiophlomis rotata

目的:考察独一味不同提取物中总环烯醚萜苷、总黄酮和总苯乙醇苷的含量。方法:独一味水提物经聚酰胺和大孔树脂通过串联及并联方法,分别富集得到水洗脱物、70%乙醇溶液洗脱物Ⅰ(70%乙醇溶液洗脱聚酰胺柱)、70%乙醇溶液洗脱物Ⅱ(70%乙醇溶液洗脱大孔树脂柱),采用一阶导数紫外分光光度法测定总环烯醚萜苷的含量;双波长紫外分光光度法、硝酸铝-亚硝酸钠显色分光光光度法测定总黄酮和总苯乙醇苷的含量。结果:建立的一阶导数紫外分光光度法测定总环烯醚萜苷的含量,在252 nm波长处加样回收率为104.40%,RSD分别为0.64%;建立的紫外分光光度法可以测定总黄酮和总苯乙醇苷的含量,总黄酮在268,363 nm波长处平均加样回收率分别为96.23%,104.48%,RSD分别为7.27%,3.61%;总苯乙醇苷在332 nm波长处加样回收率为100.26%,RSD 2.92%。结论:一阶导数紫外分光光度法测定总环烯醚萜苷的含量可行;紫外分光光度法可以测定总黄酮和总苯乙醇苷的含量。

Objective： To investigate determination of total iridoid glycoside,total flavonoids and total phenethanol glycosides from different extractive of extract of Lamiophlomis rotata. Method： Water extract of L. rotata were separated and enriched obtain water eluate and 70% ethanol solution eluateⅠ（70% ethanol solution eluted polyamide column）,70% ethanol solution eluate Ⅱ （70% ethanol solution eluting macroporous resin column） by polyamide and macroporous resin. Total iridoid glycosides ingredients were assayed by the first derivative spectrophotometry;the total flavonoids and total benzene glycosides were assayed by dual wavelength UV spectrophotometry,aluminum nitrate-sodium nitrite UV spectrophotometry. Result： Recoveries of the total iridoid glycosides was 104.40%,RSD was 0.64% at 252 nm wavelength by the first derivative UV spectrophotometry. Recoveries of the total flavonoids were 96.23% and 104.48%,RSDs were 7.27% and 3.61% at the 268 nm and 363 nm wavelengths by UV spectrophotometry. Recoveries of the total benzene glycosides was 100.26%,RSD was 2.92% at 332 nm wavelength. Conclusion： The first derivative ultraviolet spectrophotometry can determine the total iridoid glycosides. The ultraviolet spectrophotometry can determine the total flavonoids and total benzene glycosides.