HPLC测定秃叶黄皮树和黄皮树中5种有效成分的含量

Determination of Five Active Ingredient in Phellodendron chinense var Glabriusculum and P.chinense by HPLC

目的： 测定秃叶黄皮树和黄皮树中黄柏碱、木兰花碱、巴马汀、小檗碱、黄柏酮的含量，并比较不同品种黄柏药材有效成分含量的差异。 方法： 采用HPLC，Xtimate C18色谱柱（4.6 mm250 mm，5 μm），流动相乙腈（A）-0.1%磷酸水溶液（B），梯度洗脱，检测波长220 nm，流速1.0 mLmin-1，柱温30℃。 结果： 5 种成分在上述条件下分离良好。黄柏碱、木兰花碱、巴马汀、小檗碱、黄柏酮分别在0.001 7~0.07，0.002~0.08，0.000 5~0.02，0.01~0.4，0.000 5~0.02 gL-1呈良好线性关系，r分别为0.999 9，0.999 9，0.999 9，0.999 9，0.999 5，平均回收率均〉97%，（RSD〈3%，n=9）。 结论： 该方法简便、准确、重复性好，可作为秃叶黄皮树与黄皮树质量控制的方法。秃叶黄皮树中黄柏碱、木兰花碱、巴马汀、小檗碱、黄柏酮含量与黄皮树无明显差异;且已成为黄柏药材的主流商品，建议将秃叶黄皮树与黄皮树一同作为黄柏收入药典。

Objective： To determine the content of phellodendrine,magnoflorine,palmatine,berberine and obakunone in Phellodendron chinense var glabriusculum and P. chinense. Method： The determination was carried out with RP-HPLC on Xtimate C18 column （4.6 mm250 mm,5 μm）. The mobile phase was acetonitrile （A）-0.1% phosphate （B） by gradient elution and the flow rate was 1.0 mL min-1. The detection wavelength was set at 220 nm at 30℃. Result： The five components separated well under the above conditions. Phellodendrine,magnoflorine,palmatine,berberine and obakunone had good linear relationship within the ranges of 0.003 5-0.07 g L-1 （r=0.999 9）,0.004-0.08 g L-1 （r=0.999 9）,0.001-0.02 g L-1 （r=0.999 9）,0.01-0.40 g L-1 （r=0.999 5）,0.001-0.02 g L-1（r=0.999 5）. The average recoveries were greater than 97%,（RSD〈3%,n=9）. Conclusion： The method is simple,accurate and repeatable,and can be used as the quality control method of P. chinense and P. chinense var glabriusculum. There are no significant differences between P. chinense and P. chinense var glabriusculum in terms of five kinds of chemical composition content. P. chinense var glabriusculum has become mainstream products. So it is recommended that P. chinense var glabriusculum should be back into the source of Phellodendri Chinensis Cortex.