基于均匀设计法评价藜芦对人参扶正功效的影响

Evaluating the Influence of Veratrum nigrum to Panax gingseng Based on Uniform Design

目的： 考察不同配比及剂量条件下藜芦对人参扶正功效的影响。 方法： ICR小鼠按体重随机分为正常组、模型组及人参不同剂量给药组。小鼠ip环磷酰胺45 mg·kg-1，隔天1次，共3次制备免疫低下小鼠模型，动物自造模第1天起给药，1次/d，共7 d，以筛选人参有效剂量范围。再采用均匀设计法，按2因素11水平分组给药，观察不同配比及剂量的人参与藜芦合煎液对模型小鼠的免疫器官（脾脏和胸腺）指数、碳廓清能力和血常规的影响。 结果： 模型组较正常小鼠体质量、脏器指数、吞噬功能、血常规均显著下降;单用人参（0.5~8.0 g·kg-1）使脾脏指数、校正吞噬指数、WBC、RBC、Hb明显升高。均匀设计法评价藜芦对人参功效的影响。 结果： ①合煎液（人参＋藜芦0.53~8.05 g·kg-1）对免疫低下小鼠的免疫器官指数未产生明显影响;②人参∶藜芦〈100∶1时，藜芦（0.031~0.13 g·kg-1）可降低人参增强吞噬细胞功能的作用且强度与藜芦的剂量成正比，当合煎剂总剂量为3.08 g·kg-1（人参∶藜芦 9∶1）时藜芦的拮抗作用最强;③人参∶藜芦〈100∶1时，藜芦（0.034~0.13 g·kg-1）可抵消人参增高WBC，RBC的作用但不影响其量效曲线，当合煎剂总剂量分别为4.58，3.48 g·kg-1（人参∶藜芦 9∶1）时藜芦的拮抗作用最强，藜芦也可抵消人参增高Hb的作用使人参的量效曲线降低。 结论： 在药典剂量范围内，藜芦可降低或抵消人参的扶正功效，其作用强度随配比和剂量的不同而改变。

Objective： To evaluate the influence of Veratrum nigrum on Panax gingseng＇s strengthened body resistance based on uniform design. Method： ICR mice were randomly divided into seven groups,including normal control,hypoimmune model and P. gingseng treatment groups. The hypoimmune mice model was constructed by intraperitoneal injection with cyclophosphamide （45 mg kg-1,qod3）. In order to choose the dose of P. gingseng,the model mice were administered with different dose P. gingsen,qd,for 7 days.The uniform design method was used by two factors and eleven levels to investigate the thymus index,spleen index,phagocytic index,blood routine examination in hypoimmune mice. Result： P. gingseng（0.5-8.0 g kg-1） could increase the spleen index,phagocytic index,blood routine obviously in hypoimmune mice,which the. weight,immune organ index,blood routine were decreased. The influence of V. Nigrum on P. gingseng：①Immune organ index：the blend medicine（P. gingseng＋ V. nigrum=0.53-8.05 g kg-1） could not affect the thymus index and spleen index obviously. ② Carbon clearance ability：when the rates lowered 100：1 between P. gingseng and V. nigrum, V. nigrum（0.031-0.13 g kg-1） could decrease phagocytosis functions of P. gingseng and the effect was changed within the rate and dose of them. When the total dose was 3.08 g kg-1（P. gingseng：V. nigrum 9：1）,V. nigrum showed the most powerful antagonistic action on Gingseng. ③Blood routine examination：when the rates lowered 100：1 between P. gingseng and V. nigrum,V. nigrum（0.034-0.13 gkg-1） could counteract P. gingseng＇s raising the WBC,RBC but could not affect P. gingseng＇s dose-response curve. When the total dose was 4.58,3.48 g kg-1respectively （P. gingseng：V. nigrum 9：1）,V. nigrum showed the most powerful antagonistic action on P. gingseng. V. Nigrum not only could counteract P.Gingseng＇s raising the Hb but also reduce Gingseng＇s dose-response curve. Conclusion： In the dosage range of Chinese Pharmacopoeia,V. nigrum could decrease even counteract the effects of P. gingseng＇s strengthen body resistance obviously,the degree of which changed within the rate and dose of them.