逍遥散对雷公藤致大鼠肝损伤的预防作用及机制探讨

Preventive Effect and Mechanism of Xiaoyao San on Liver Injury Induced by Tripterygium wilfordii

目的:研究逍遥散对雷公藤水煎剂致大鼠急性肝损伤的预防作用及其作用机制。方法:60只SD大鼠随机分为正常对照组、模型组、甘利欣(0.06 g·kg-1)对照组、逍遥散低、中、高剂量组(生药量3.38,6.75,13.50 g·kg-1)。除正常组外,分别以相应剂量药物连续ig 4 d,再以雷公藤水煎剂(生药量3.75 g·kg-1)ig 4 d造模。处死大鼠,计算肝指数,检测血清丙氨酸转氨酶(ALT)和天冬氨酸转氨酶(AST)的活力,做肝脏病理学检查,并测定肝匀浆上清白细胞介素1β(IL-1β)和肿瘤坏死因子α(TNF-α)的含量。结果:与正常组比较,模型组大鼠的肝指数及血清ALT,AST的活力明显升高(P<0.01),肝组织病理改变明显(P<0.05),肝匀浆上清IL-1β,TNF-α的含量明显升高(P<0.05);与模型组比较,逍遥散各组和甘利欣组大鼠的肝指数、血清ALT和AST的活力、肝组织病理损伤程度及肝匀浆上清IL-1β,TNF-α的含量均明显降低(P<0.05或P<0.01)。结论:雷公藤所致大鼠急性肝损伤与其引起炎症因子IL-1β,TNF-α释放增加进而形成炎性损伤有关,逍遥散可通过抑制IL-1β,TNF-α的释放而对雷公藤致大鼠急性肝损伤起到预防作用。

Objective： To study the preventive effect and mechanism of Xiaoyao San on rat acute liver injury induced by Tripterygium wilfordii. Method： Sixty rats were randomly divided into control group, T. wilfordii model group, ganlixin group, low-dose, medium-dose and high-dose groups of Xiaoyao San. Each group was respectively fed with the corresponding dose of drug for 4 days and then fed with decoction of T. wilfordii for 4 days to make the model except control group. Liver indexes was calculated, alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, interleukin-1β（IL-1β）, tumor necrosis factor（TNF）-αwere measured, and liver pathology was detected. Results： Compared with control group, liver indexes, ALT, AST, IL-1β and TNF-α in model group were significantly increased, and significant pathological changes of liver tissue were found（P〈0.05 or P〈0.01）. Compared with model group, all doses group of Xiaoyao San and ganlixin group could effectively decrease liver indexes, ALT, AST, IL-1β, TNF-α and pathological changes of liver tissue （P〈0.05 or P〈0.01）. Compared with ganlixin group,liver indexes, ALT, AST, IL-1β, TNF-α and pathological changes of liver tissue of all doses group of Xiaoyao San was not significantly different. Conclusion： T. wilfordii-induced acute liver injury is associated with inflammatory injury via IL-1β and TNF-α. Xiaoyao San can protect the liver injury induced by T. wilfordii by inhibiting inflammatory injury.