荞麦黄酮对2型糖尿病胰岛素抵抗大鼠炎症因子的影响

Effects of Flavones of Buckwheat Flower and Leaf on Inflammation Factors in Rats with Type 2 Diabetes Insulin Resistance

目的:探讨荞麦黄酮(FB)对2型糖尿病(T2DM)大鼠糖脂代谢、胰岛素抵抗(IR)和炎症因子的影响及机制。方法:SD大鼠以高脂饮食喂养联合小剂量链脲佐菌素(STZ)腹腔注射制备T2DM大鼠模型。将大鼠随机分为正常对照组,模型组,阳性对照组(罗格列酮1 mg·kg-1,ig),FB低、中、高剂量组(FB100,200,400 mg·kg-1,ig)。测定空腹血糖(FBG)、血清总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)及低密度脂蛋白胆固醇(LDL-C);放免法测定血清空腹胰岛素(FINS),计算胰岛素敏感指数(ISI);酶联免疫法测定肿瘤坏死因子-α(TNF-α)、白介素-6(IL-6)、C-反应蛋白(CRP)。结果:模型组大鼠较正常对照组血清FBG,TC,TG,LDL-C,FINS,TNF-α,IL-6,CRP均显著升高(P<0.01),HDL-C显著下降(P<0.01);给予FBFL干预后糖脂代谢各指标明显改善,炎症因子TNF-α、IL-6、CRP水平明显降低(P<0.01,P<0.05),并呈一定剂量依赖性。结论:FB可能通过降低血清炎症因子TNF-α,IL-6,CRP水平,改善2型糖尿病大鼠糖脂代谢紊乱、胰岛素抵抗。

Objective： To investigate the effects of flavones of buckwheat （FB） on blood glucose and lipids level,insulin resistance and inflammation factors in Rats with Type 2 Diabetes. Method： Male SD rats were fed high-fat diet after 4 weeks,then injected small dose streptozotocin （STZ） to establish animal models of type 2 diabetes. The rats were divided into 6 groups：normol group,model group,rosiglitazone group（RSg,1 mgkg-1,ig）,low,middle and high dosage-FB group （100,200,400 mg kg-1,ig）. After 4 weeks＇ treatment,the levels of FBG,TC,TG,LDL-C,HDL-C,FINS,TNF-α,IL-6,CRP were detected,and insulin sensitive index （ISI） was calculated. Result： Compared with the normol group,levels of serum FBG,TC,TG,LDL-C,FINS,TNF-α,IL-6 and CRP in model group were increased（P〈0.01）,but the levels of HDL-C and ISI were decreased significantly（P〈0.01）. In FB groups,levels of FBG,TC,TG,LDL-C,FINS,TNF-α,IL-6 and CRP were reduced compared with model group（P 〈0.01,P 〈0.05）,while HDL-C and ISI were increased in a dose-dependent manner. Conclusion： FB can effectively improve glucose and lipid disorders,improve insulin resistance possibly by decreasing the serum levels of inflammatory cytokines such as TNF-α,IL-6 and CRP of type2 diabetes mellitus rats.