晚期胃肠道肿瘤血瘀程度与外周血肿瘤缺氧相关蛋白关系

On Relation of Blood Stasis and Serum Hypoxia-associated Proteins in Patients with Advanced Gastroenteric Cancer

目的:探讨晚期胃肠道肿瘤血瘀程度与外周血肿瘤缺氧相关蛋白之间的关系。方法:选取本院肿瘤科2012年4月1日到2013年4月10日IV期胃癌、十二指肠癌、结直肠癌患者46例,另选取同期住院的同类疾病术后无瘤患者29例,本院体检中心健康体检者19例作为对照,入院次日抽取空腹静脉血,采用ELISA法检测血管内皮生长因子(VEGF)、骨桥蛋白(OPN)、碳酸酐酶IX(CAIX)水平。结果:晚期肿瘤以及术后无瘤组患者外周血VEGF,OPN,CAIX水平均明显高于健康对照组,晚期肿瘤组与术后无瘤组之间,前者VEGF,OPN,CAIX分别是后者的1.27倍(P>0.05)、1.33倍(P<0.05)、1.48倍(P>0.05);晚期肿瘤血瘀程度重组VEGF,CAIX水平明显高于血瘀程度轻组,前者是后者的1.92倍(P<0.01)、2.70倍(P<0.01);血瘀证症候脉络瘀血、癥积程度与VEGF,OPN,CAIX水平相关,程度越重,缺氧相关蛋白水平越高。结论:晚期胃肠道肿瘤血瘀证与外周血缺氧相关蛋白VEGF,OPN,CAIX相关。

Objective： The purpose of this study was to investigate the relationship between the extent of blood stasis and hypoxia-related proteins in the advanced gastrointestinal cancer patients. Method： We recruited 46 patients with stage IV advanced gastrointestinal cancer,29 patients after radical resection of digestive tract tumor and 19 health adults as control between April 1,2012 and April 10,2013.Fasting venous blood were extracted in hospital the next day,and ELISA test were performed to examine the expression level of vascular endothelial growth factor（VEGF）,osteopont in（OPN） and carbonic anhydrase IX（CAIX）. Result： The patients with advanced cancer,and postoperative cancer group peripheral blood level of VEGF and OPN,CAIX were significantly higher than that of healthy controls. Between advanced cancer group and postoperative cancer group,the former of VEGF and OPN,CAIX respectively was 1.27 times（P〉0.05）,1.33 times（P〈0.05）,1.48 times（P〉0.05） of the latter.In the advanced cancer group,the level of VEGF、CAIX in the severe blood stasis group were significantly higher than the mild group. The former respectively was 1.92 times（P〈0.01）,2.70 times（P〈0.01） of the latter.The extent of blood stasis and blood stasis symptoms degree were related to the level of VEGF and OPN,CAIX. The heavier degree of blood stasis,the higher level of anoxia related protein. Conclusion： The expression of hypoxia-related proteins increased gradually with the blood stasis degree in the advanced gastrointestinal cancer patients.