miR-152对前列腺癌细胞株迁移和侵袭能力的影响

Effect of miR-152 on migration and invasion of prostate cancer cell lines

目的研究miR-152在前列腺癌、前列腺正常组织中的表达情况及其在前列腺癌细胞系中的作用。方法采用TaqMan荧光定量RT-PCR方法检测8例前列腺癌和8例前列腺正常组织的样本中miR-152的表达水平。运用Transwell细胞迁移实验及侵袭实验评估miR-152对前列腺细胞系PC-3和DU145细胞功能的影响。结果与正常前列腺组织相比,miR-152在前列腺癌组织中的表达水平显著下调(P<0.05)。体外实验中上调miR-152的表达可以显著降低前列腺癌细胞的迁移和侵袭能力(P<0.05)。结论 miR-152在前列腺癌中可作为一种肿瘤抑制因子,影响前列腺癌细胞的迁移和侵袭能力。

Objective To investigate the effect of miR-152 on the migration and invation of prostate cancer （PCa） cell lines. Methods The miR-152 expressions in 8 cases of primary PCa and 8 samples of non-malignant prostatic tissue were measured by qRT-PCR. The effects of miR-152 expression on PCa cells were evaluated by Transwell cell migration and invasion assays. Results The miR-152 expression was significantly decreased in primary PCa samples compared with that in non-malignant samples （P〈O. 05）. The miR-152 expression significantly reduced the migratory and invasive capabilities of PCa cells in vitro （P〈0.05）.Conlclusions Our findings indicate that miR-152 can act as a tumor suppressor and inhibit PCa cell migration and invasion.