应用外周血CD34^+细胞与骨髓间充质干细胞共培养细胞膜片修复兔颅骨缺损

Treatment of a rabbit calvarial critical-size defect using co-cultured peripheral blood CD34^+ cell and bone marrow-derived mesenchymal stem cell sheet

目的:评价外周血CD34+细胞(PB-CD34+Cs)与骨髓间充质干细胞(BM-MSCs)共培养所得细胞修复兔颅骨极限缺损的能力。方法:分离PB-CD34+Cs及BM-MSCs,建立2种细胞的共培养体系。将共培养细胞诱导为膜片,复合羟基磷灰石支架材料修复兔颅骨直径15 mm的圆形缺损(n=10),并设置单纯BM-MSCs组(n=10)及空白对照(n=5)。术后4、6、8周行CT扫描,术后8周取材,行组织学检查,评价骨修复效果。结果:成膜诱导14 d后获得细胞膜片,膜片具有良好的韧性和可操作性。影像学及组织学定量检测结果显示,共培养细胞组颅骨缺损的修复效果最佳,明显优于单纯BM-MSCs对照组及空白对照组。结论:共培养细胞有效地修复了兔颅骨极限缺损,其效果优于BM-MSCs。

Objective：To evaluate the efficacy of peripheral blood CD34 ＋ cells（PB-CD34 ＋ Cs） co-cultured with bone marrow-derived mesenchymal stem cells （BM-MSCs） in repairing rabbit calvarial critical-size defect.Methods：PB-CD34 ＋ Cs and BM-MSCs were co-cultured and induced into cell sheet.The co-cultured cell sheet was composited with hydroxyapatite and used to repair a 15mm-diameter calvarial circular defect in 10 rabbits.BM-MSCs control（n =10） and blank control（n =5） were set up at the same time.CT scan was performed 4,6 and 8 weeks after surgery respectively.Samples were harvested 8 weeks after operation.The new bone formation was investigated by histological examination.Results：Cell sheets were acquired after 14 days of induction.The treatment outcome in co-cultured cell group was better than that in the two control groups evaluated by either imagining or quantitative histological examination.Conclusion：Co-cultured PB-CD34＋ Cs and BM-MSCs may promote bone formation in calvarial defect and are more effective than BM-MSCs.