小鼠小脑皮质发育过程中细胞迁移及血管之间的相互作用

Interaction between cell migration and vasculature in the developing cerebellum of the mouse

目的探讨小鼠小脑片层化过程中血管发生与细胞迁移之间的关系。方法不同年龄小鼠共计146只,应用免疫荧光法和5'-溴脱氧尿嘧啶核苷(BrdU)法及墨汁血管灌注技术,标记小鼠胚胎期10日(E10)至出生90日(P90)小脑的放射状胶质细胞,普肯耶细胞,颗粒细胞以及小脑内血管发生。结果在E15左右,小脑皮质内开始出现血管网;尔后随着年龄的增长,放射状胶质细胞和血管之间相互诱导,血管开始变得密集,我们可以观察到,外颗粒层的血管沿着放射状胶质细胞分布,但是在内颗粒层和白质,血管的走行比较紊乱,和放射状胶质细胞走行保持高度一致。结果还发现很多BrdU阳性细胞紧贴着血管迁移。结论在小脑片层化形成的过程中,细胞迁移起着非常关键的作用,血管在小脑皮质内不仅和放射状胶质细胞相互作用,引导神经细胞的迁移,并且为神经细胞的迁移提供路径和支架。

Objective Our aim was to explore the interaction between angiogenesis and neural migration in the developing cerebellum of mice. Methods The immunofluorescence, BrdU and ink perfusion were used to label the radial glial cells, Purkinje cells, granule ceils and vasculature in the mouse cerebellum aged from El0 （embryonic day 10） to P90 （postnatal day 90）. A total of 146 mice at various ages were used in the study. Results Vascular network began to appear in the cerebellum at about El5. Tith age increasing, vascular density increased. In the meantime, there was close relationship between the radial glial cell development and vascular development. For instance, blood vessels and the projections of radial glial cells in the external granular layer were arranged regularly and paralleled each other, but the distributions of vessels and radial glial fibers in the internal granular layer and white matter were in disorder, suggesting the interaction between the vasculature and radial glial cells. Finally, the study also showed that many BrdU-positive cells migrated along blood vessels. Conclusion Cell migration plays a crucial role in cerebellar lamellae formation process. We found that the blood vessels not only have interaction with the radial glial cells, but also guide the migration of neurons in the cerebellum, probably serving as scaffolds for the neuronal migration.