热敏灸对骨质疏松症大鼠筋膜形态改变的研究

Study of thermal moxibustion on morphological change of the fascia in osteoporosis rats

目的为研究筋膜中结缔组织在热敏灸信号传导中的作用,揭示热敏灸的普遍机理。方法选取80只SD雌性大鼠随机分为正常组,模型组、艾灸组及热敏灸组各20只,并对后三组进行造模使其处于骨质疏松症病理状态下,然后对艾灸组及热敏灸组取肾俞穴进行常规艾灸及热敏灸治疗,并取其灸点周围结缔组织进行染色,观察各组成纤维细胞形态的改变。结果热敏灸组ECM内纤维以灸点为中心呈向心性分布、张力增高,其他三组纤维纵横交错形成众多纤维网格。热敏灸组细胞在纤维带动下排列方向一致、单位面积内细胞密度较大,在纤维拉动下,细胞骨架重构成"扁梭形"、胞浆纵轴长而致密、细胞突起界限不清,胞核小、呈长梭形,其他三组大量细胞杂乱无序填充在纤维网格内、单位面积内细胞密度较小、细胞分界清晰,胞内骨架伸展成"片状"、多突起并相互形成联系,胞核近似圆形位于胞浆中心。结论实施热敏灸时,灸刺激能通过使周围大量成纤维细胞可逆性收缩而到达整个间隙结缔组织。

Objective To investigate the effect of fascial connective tissue in the thermal moxibustion signal transduetion, and to reveal the universial mechanism of thermal moxibustion. Methods Eighty female SD rats were randomly divided into normal group, model group, moxibustion group, and thermal moxibustion group, and each group had 20 rats. Rats in the latter 3 groups were modeled in osteoporosis pathological state. Rats in moxibustion group and thermal moxibustion group were given conventional moxibustion and thermal moxihustion treatment at the Shenshu acupoint, respectively. Then the connective tissue around the moxibustion point was stained, and the morphological change of fibroblasts in each group were observed. Results In thermal moxihustion group, fibers in ECM centripetally distributed around the moxihustion point, with increased tension, while fibers in the other 3 groups crossed irregularly and formed numerous fribrous gridding. Cells in thermal moxibustion group arranged in the same dirrection under the traction of fibers. The density of cells in a unit area was high. Under the traction of fibers, cytoskeleton was reconstructed into flat spindle shape. The vertical axis was long and dense. The bound of cellular processes was unclear, and the nuclear was small and long spindle shaped. Cells in the other 3 groups filled in the fibrous griddings disorderly. The cell density was much lower. And the bound- of cells was clear. The cytoskeleton streched into flake, with abundant processes and connected with each other. The nucleus was aprroximate round and located in the center of the cytoplasma. Conclusion When implementating thermal moxibustion, moxibustion stimulation can reach the whole connective tissue through the reversible contraction of abundant fibroblasts around the moxibustion point.