籼粳稻及恢复系Rf-1位点PCR片段的序列分析

Analysis of PCR Sequences on Rice Rf-1 Locus of indica, japonica and Restorer Lines

水稻育性恢复基因是鉴别籼粳亚种的关键基因。本研究利用育性恢复基因Rf-1位点上的特异性引物68923-8对来源不同的籼、粳稻恢复系及非恢复系进行PCR片段的序列比对及遗传距离分析。结果如下:在籼稻中,非恢复系间的一致性为94.97%,恢复系间的一致性为88.03%,恢复系与非恢复系之间的一致性达到91.56%;粳稻中,非恢复系间的一致性为91.56%,恢复系间一致性为57.48%,但恢复系与非恢复系间的一致性却较低,为36.53%;比较籼粳稻之间发现,二者非恢复系间的一致性为57.66%,恢复系间的一致性为54.71%。遗传距离的分析也揭示与此相似的结果。聚类将供试材料分成三大枝:第一大枝包含籼稻恢复系、籼稻非恢复系及1个粳稻恢复系Ansanbyeo;第二大枝包含6个粳稻非恢复系;而滇Ⅰ型恢复系南34与BT型恢复系C418则单独成第三大枝,而且与其它两枝的同源性较低。结果表明:水稻籼-粳亚种的遗传变异反映在育性恢复基因Rf-1位点的DNA序列变异上。

Fertility restorer gene （RD is a key gene to identify indica and japonica rice. In the present study, sequ- ences of PCR products by specific primer 68923-8 on Rf-1 locus of indica, japonica restorer and non-restorer lines in rice were analyzed. Results showed： in indica, genetic identity among indica non-restorers and restorers was 94.97% and 88.03%, respectively, and it was 91.56% between restorers and non-restorers; in japonica, The identity was 91.56% and 57.48% among japonica non-restorers and japonica restorers, respectively, and it was rather low, only 36.53% between non-restorers and restorers. The identity was 57.66% between indica and japonica lines of non-restorers, and it was 54.71% between indica-japonica lines of restorers. Analysis based on genetic distances gave the similar results. Cluster analysis showed~ all the materials divided into 3 main branches, the first main branch had 7 indica materials and 1 japonica restorer line from South Korea. The second main branch had 6 japonica non-restorers. And Nan34 and C418 got together in the third main branch, which had low identity with the other two branches. The results indicated that DNA sequences variation of Rf-1 locus reflected genetic differ- entiation ofindica-japonica subspecies.